Integrating multi-locus genome-wide association studies with transcriptomic data to identify genetic loci underlying adult root trait responses to drought stress in flax (Linum usitatissimum L.)

Simple item page
datacite.alternateIdentifier.citationEnvironmental and Experimental Botany, 202, 2022
datacite.alternateIdentifier.doi10.1016/j.envexpbot.2022.105019
datacite.alternateIdentifier.issn0098-8472
datacite.creatorSoto-Cerda, Braulio Jorge
datacite.creatorLarama, Giovanni A.
datacite.creatorGajardo, Humberto A.
datacite.creatorInostroza-Blancheteau, Claudio
datacite.creatorCloutier, Sylvie J.
datacite.creatorFofana, Bourlaye
datacite.creatorAbanto Marin, Michel
datacite.creatorAravena, Gabriela A.
datacite.date2022
datacite.rightsRegistro bibliográfico
datacite.subjectCandidate Genes
datacite.subjectDrought Stress
datacite.subjectLinum Usitatissimum
datacite.subjectMulti-locus Gwas
datacite.subjectRna-seq
datacite.subjectRoot Traits
datacite.subjectCrop Production
datacite.subjectDicotyledon
datacite.subjectDrought Stress
datacite.subjectGenetic Analysis
datacite.subjectGenome
datacite.subjectIntegrated Approach
datacite.subjectPhenotype
datacite.subjectRna
datacite.subjectRoot-shoot Ratio
datacite.titleIntegrating multi-locus genome-wide association studies with transcriptomic data to identify genetic loci underlying adult root trait responses to drought stress in flax (Linum usitatissimum L.)
dc.contributor.authorSOTO CERDA, BRAULIO JORGE
dc.contributor.authorSOTO CERDA, BRAULIO JORGE
dc.contributor.authorINOSTROZA BLANCHETEAU, CLAUDIO ANDRES
dc.description.abstractWater stress is a major constraint on crop production and, root systems are one of the key features of plants determining drought tolerance and yield. In this study, five root morphological traits and their stability indices were evaluated at the reproductive stage on an association panel consisting of 120 diverse Linum usitatissimum accessions grown under well-watered and water-limiting conditions. To identify quantitative trait loci (QTL) and candidate genes positively influencing root traits under drought, combined multi-locus genome-wide association studies (ML-GWAS) and transcriptomic data analysis were carried out. A total of 112 QTL were identified accounting for 0.45 24.28 % of the phenotypic variation for root-related traits. Fifty QTL were detected by at least two ML-GWAS models, and 26 were co-located with previously reported QTL associated with yield-related traits under drought and early root and shoot development. Phenotypic differences between two contrasting subsets of accessions for the traits assessed were attributable to the accumulation of positive QTL alleles (PQTL), where the subset with the largest number of PQTL registered 154 % and 69 % greater total root length and yield under drought stress, respectively, than the subset with the fewest PQTL. Transcriptomic data analysis identified 766 differentially expressed genes (DEGs) responsive to drought stress in 108 QTL. Annotation of the 766 DEGs yielded functional genes involved in root development, stomatal closure, ROS (reactive oxygen species) scavenging, and hormone responses, among others. Taken together, the combined use of GWAS and transcriptomic data analyses provide the foundation for our enhanced understanding of root trait morphological changes in response to drought stress, and to achieve superior yield resilience/stability under water shortages in flax through enhanced molecular breeding. Data availability: All data supporting the findings of this study are available within the paper and within its Supplementary data © 2022 Elsevier B.V., All rights reserved.
dc.description.ia_keywordroot, drought, data, were, stress, traits, under
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4836
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceEnvironmental and Experimental Botany
dc.subject.ia_odsODS 6: Agua limpia y saneamiento
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2022
oaire.citationTitleEnvironmental and Experimental Botany
oaire.citationVolume202
oaire.fundingReferenceANID FONDECYT 1161133 (Regular)
oaire.fundingReferenceUniversidad Católica de Temuco
oaire.fundingReferenceAgriculture and Agri-Food Canada (AAFC)
oaire.fundingReferenceGenome Canada TUFGEN
oaire.licenseConditionCopyright © Elsevier B.V., 2022
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication8e8b0607-b16f-4744-887c-bb6b4544a8a8
relation.isAuthorOfPublication8e8b0607-b16f-4744-887c-bb6b4544a8a8
relation.isAuthorOfPublication25287ee1-cae8-401d-b42c-ee3f8fc05d2a
relation.isAuthorOfPublication.latestForDiscovery8e8b0607-b16f-4744-887c-bb6b4544a8a8
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionCAB Abstracts
Files
Collections
Producción Alimentaria