Effect of human tubal fluid medium and hyperactivation inducers on stallion sperm capacitation and hyperactivation

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datacite.alternateIdentifier.citationReproduction in Domestic Animals, 54 (2), 194-184, 2019
datacite.alternateIdentifier.doi10.1111/rda.13328
datacite.alternateIdentifier.issn1439-0531
datacite.creatorArroyo-Salvo, Camila A.
datacite.creatorSanhueza, Francisco
datacite.creatorFuentes, Fernanda B.
datacite.creatorTreulén, Favián
datacite.creatorÁrias, María Elena
datacite.creatorCabrera, Paulina
datacite.creatorSilva, Mauricio Erciario
datacite.creatorFelmer, Ricardo N.
datacite.date2019
datacite.rightsRegistro bibliográfico
datacite.subjectCapacitation
datacite.subjectHyperactivation
datacite.subjectProcaine
datacite.subjectSperm
datacite.subjectStallion
datacite.subjectCaffeine
datacite.subjectProcaine
datacite.subjectAminopyridines
datacite.subjectCaffeine
datacite.subjectCulture Media
datacite.subjectFertility Agents, Male
datacite.subjectFluorescein Isothiocyanate-peanut Agglutinin
datacite.subjectFluoresceins
datacite.subjectPeanut Agglutinin
datacite.subjectProcaine
datacite.subjectAminopyridine Derivative
datacite.subjectCaffeine
datacite.subjectFertility Promoting Agent
datacite.subjectFluorescein Derivative
datacite.subjectFluorescein Isothiocyanate-peanut Agglutinin
datacite.subjectPeanut Agglutinin
datacite.subjectProcaine
datacite.subjectAcrosome Reaction
datacite.subjectAnimal
datacite.subjectCulture Medium
datacite.subjectDrug Effect
datacite.subjectHorse
datacite.subjectIn Vitro Fertilization
datacite.subjectMale
datacite.subjectMitochondrial Membrane Potential
datacite.subjectPharmacology
datacite.subjectPhosphorylation
datacite.subjectProcedures
datacite.subjectSemen Analysis
datacite.subjectSperm
datacite.subjectSpermatozoon
datacite.subjectSpermatozoon Capacitation
datacite.subjectSpermatozoon Motility
datacite.subjectVeterinary Medicine
datacite.subjectAcrosome Reaction
datacite.subjectAminopyridines
datacite.subjectAnimals
datacite.subjectCaffeine
datacite.subjectCulture Media
datacite.subjectFertility Agents, Male
datacite.subjectFertilization In Vitro
datacite.subjectFluoresceins
datacite.subjectHorses
datacite.subjectMale
datacite.subjectMembrane Potential, Mitochondrial
datacite.subjectPeanut Agglutinin
datacite.subjectPhosphorylation
datacite.subjectProcaine
datacite.subjectSemen
datacite.subjectSemen Analysis
datacite.subjectSperm Capacitation
datacite.subjectSperm Motility
datacite.subjectSpermatozoa
datacite.titleEffect of human tubal fluid medium and hyperactivation inducers on stallion sperm capacitation and hyperactivation
dc.contributor.authorSILVA JIMENEZ, MAURICIO
dc.description.abstractConventional in vitro fertilization has not yet been implemented in the equine species. One of the main reasons has been the inability to develop a culture medium and incubation conditions supporting high levels of stallion sperm capacitation and hyperactivation in vitro. Although different culture media have been used for this purpose, human tubal fluid (HTF) medium, widely used in the manipulation of human and mice gametes, has not been reported so far in stallion sperm culture. The first part of this study aimed to compare HTF and Whitten's media on different stallion sperm quality and capacitation variables. Additionally, the effect of procaine, aminopyridine and caffeine in both media was evaluated on sperm motility parameters at different incubation times. Integrity and destabilization of the plasma membrane were evaluated by merocyanine 540/SYTOX Green (MC540), mitochondrial membrane potential (??m) using tetramethylrhodamine methyl ester perchlorate (TMRM), acrosome membrane integrity by PNA/FITC and tyrosine phosphorylation by P-tyrosine mouse mAb conjugated to Alexa Fluor® by flow cytometry. Motility parameters were evaluated using the integrated semen analysis system (ISAS®). We found no differences between Whitten's and HTF media and incubation time in terms of sperm viability, uninduced acrosome membrane damage or mitochondrial membrane potential at 30- and 120-min incubation. Membrane fluidity (MC540) increased in both media at 30- and 120-min incubation compared to noncapacitating conditions. Similarly, tyrosine phosphorylation increased in both media in capacitating conditions at 2- and 4-hr incubation compared to noncapacitating conditions. Although procaine showed the best result in terms of sperm hyperactivated motility in both media, aminopyridine also showed parameters consistent with the hyperactivation including an increase in curvilinear velocity and decrease in straightness. In conclusion, HTF medium and aminopyridine equally support capacitation-related parameters in stallion sperm. © 2019 Elsevier B.V., All rights reserved.
dc.description.ia_keywordsperm, media, incubation, membrane, stallion, medium, conditions
dc.identifier.issn0936-6768
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3498
dc.language.isoen
dc.publisherWiley
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceReproduction in Domestic Animals
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nEcología
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2019
oaire.citationEndPage194
oaire.citationIssue2
oaire.citationStartPage184
oaire.citationTitleReproduction in Domestic Animals
oaire.citationVolume54
oaire.fundingReferenceANID FONDECYT 1160467 (Regular)
oaire.fundingReferenceUniversidad de La Frontera Dirección de Investigación
oaire.licenseConditionCopyright © Blackwell Verlag GmbH, 2018
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication5a5fa19b-bce1-4387-8139-279b35112838
relation.isAuthorOfPublication.latestForDiscovery5a5fa19b-bce1-4387-8139-279b35112838
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionCAB Abstracts
uct.indizacionVeterinary Bulletin
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