Antifungal Effects of Drimane Sesquiterpenoids Isolated from Drimys winteri against Gaeumannomyces graminis var. tritici

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datacite.alternateIdentifier.citationApplied and Environmental Microbiology, 86 (24), 10-1, 2020
datacite.alternateIdentifier.doi10.1128/AEM.1834-20
datacite.alternateIdentifier.issn1098-5336
datacite.creatorPaz, Cristian
datacite.creatorViscardi, S.
datacite.creatorIturra, Andres
datacite.creatorMarin, Victor
datacite.creatorMiranda, Felipe
datacite.creatorBarra, P. J.
datacite.creatorMéndez, Isabel
datacite.creatorDurán, Paola C.
datacite.date2020
datacite.rightsAcceso abierto
datacite.subjectAntifungal Agents
datacite.subjectMembrane Disruption
datacite.subjectNatural Products
datacite.subjectTake-all Disease
datacite.subjectAntifungal Agents
datacite.subjectDrimane
datacite.subjectPolycyclic Sesquiterpenes
datacite.subjectSesquiterpenes
datacite.subjectAgricultural Robots
datacite.subjectAgriculture
datacite.subjectAntifungal Agents
datacite.subjectDisease Control
datacite.subjectFourier Transform Infrared Spectroscopy
datacite.subjectFungi
datacite.subjectLipids
datacite.subjectMedical Applications
datacite.subjectMetabolites
datacite.subjectOxidation
datacite.subjectOxidative Stress
datacite.subjectPathogens
datacite.subjectPurification
datacite.subjectThin Layer Chromatography
datacite.subjectAnti-fungal Properties
datacite.subjectAnti-inflammatory Activity
datacite.subjectAnti-microbial Effects
datacite.subjectGaeumannomyces Graminis
datacite.subjectGaeumannomyces Graminis Var. Tritici
datacite.subjectPhytopathogenic Fungi
datacite.subjectSecondary Metabolites
datacite.subjectSustainable Agriculture
datacite.subjectValue Engineering
datacite.subjectAntimicrobial Activity
datacite.subjectBiotechnology
datacite.subjectBiotic Factor
datacite.subjectChemical Control
datacite.subjectFungal Disease
datacite.subjectFungus
datacite.subjectPathogen
datacite.subjectSoilborne Disease
datacite.subjectWheat
datacite.subjectChile
datacite.subjectDrimys Winteri
datacite.subjectHexapoda
datacite.subjectAntifungal Agent
datacite.subjectDrimane
datacite.subjectSesquiterpene
datacite.subjectAscomycetes
datacite.subjectBark
datacite.subjectCell Wall
datacite.subjectChemistry
datacite.subjectDrimys
datacite.subjectDrug Effect
datacite.subjectLipid Peroxidation
datacite.subjectPharmacology
datacite.subjectAntifungal Agents
datacite.subjectAscomycota
datacite.subjectCell Wall
datacite.subjectLipid Peroxidation
datacite.subjectPlant Bark
datacite.subjectPolycyclic Sesquiterpenes
datacite.subjectSesquiterpenes
datacite.titleAntifungal Effects of Drimane Sesquiterpenoids Isolated from Drimys winteri against Gaeumannomyces graminis var. tritici
dc.contributor.authorVISCARDI, SHARON
dc.date.accessioned2025-10-06T14:21:41Z
dc.date.available2025-10-06T14:21:41Z
dc.description.abstractGaeumannomyces graminis var. tritici is a soilborne pathogen that causes take-all disease, affecting cereal roots. In wheat, G. graminis var. tritici is the most important biotic factor, causing around 30 to 50% losses of yield. Chemical control of this fungal disease is difficult because G. graminis var. tritici is able to reside for a long time in soils. Therefore, the development of environmentally friendly biotechnological strategies to diminish the incidence of soilborne diseases is highly desirable. Natural products are a promising strategy for biocontrol of plant pathogens. A special emphasis is on medicinal plants due to their reported fungitoxic effects. Drimys winteri (canelo) is a medicinal plant that is widely used by the Mapuche ethnic group from Chile due to its anti-inflammatory activity. In addition, inhibitory effects of canelo against phytopathogenic fungi and pest insects have been reported. In this study, we isolated, purified, and identified six drimane sesquiterpenoid compounds from canelo (drimenin, drimenol, polygodial, isodrimeninol, valdiviolide, and drimendiol). Then, we evaluated their antimicrobial effects against G. graminis var. tritici. Compounds were identified by comparing Fourier-transform infrared spectroscopy (FTIR) data and the retention time in thin-layer chromatography (TLC) with those of pure standards. The putative antagonistic effects were confirmed by assessing hyphal cell wall damage using confocal microscopy and lipid peroxidation. Here, we reported the high potential of drimane sesquiterpenoids as natural antifungals against G. graminis var. tritici. Polygodial and isodrimeninol were the most effective, with 50% lethal concentrations (LC<inf>50</inf>s) between 7 and 10 µg ml?1 and higher levels of fungal lipid peroxidation seen. Accordingly, natural sesquiterpenoids purified from canelo are biologically active against G. graminis var. tritici and could be used as natural biofungicides for sustainable agriculture. IMPORTANCE More than two billion tons of pesticides are used every year worldwide. An interesting sustainable alternative to control plant pathogens is the use of natural products obtained from plants, mainly medicinal plants that offer secondary metabolites important to human/animal health. In this study, we isolated and identified six pure drimane sesquiterpenoids obtained from the bark of Drimys winteri. Additionally, we evaluated their antifungal activities against Gaeumannomyces graminis (the main biotic factor affecting cereal production, especially wheat) by assessing fungal cell wall damage and lipid peroxidation. The compounds obtained showed important antifungal properties against G. graminis var. tritici, mainly isodrimenol, which was the second-most-active compound after polygodial, with an LC<inf>50</inf> against G. graminis var. tritici of around 9.5 µg ml?1. This information could be useful for the development of new natural or hemisynthetic antifungal agents against soil-borne phytopathogens that could be used in green agriculture. © 2020 Elsevier B.V., All rights reserved.
dc.description.ia_keywordgraminis, tritici, against, natural, effects, canelo, drimane
dc.formatPDF
dc.identifier.issn0099-2240
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6743
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceApplied and Environmental Microbiology
dc.subject.ia_odsODS 3: Salud y bienestar
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2020
oaire.citationEndPage10
oaire.citationIssue24
oaire.citationStartPage1
oaire.citationTitleApplied and Environmental Microbiology
oaire.citationVolume86
oaire.fundingReferenceANID FONDECYT REGULAR 1201196
oaire.fundingReferenceANID FONDECYT INICIACIÓN 11181076
oaire.fundingReferenceANID INACH RT06-17
oaire.licenseConditionObra bajo licencia Creative Commons Atribución-No Comercial 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by-nc/4.0/
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublicationae819263-1d82-40a8-b52a-d2ed58a7aed0
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uct.catalogadorjvu
uct.comunidadCiencias de la Saluden_US
uct.departamentoDepartamento de Procesos Diagnósticos y Evaluación
uct.facultadFacultad de Ciencias de la Salud
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed Central (PMC)
uct.indizacionScimago
uct.indizacionPubMed
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