Role of three plasma membrane Ca2+-binding proteins in the sperm motility of Atlantic salmon (Salmo salar)

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datacite.alternateIdentifier.citationAquaculture, 538, 2021
datacite.alternateIdentifier.doi10.1016/j.aquaculture.2021.736537
datacite.alternateIdentifier.issn0044-8486
datacite.creatorBeltran, Jorge F.
datacite.creatorFigueiras-Fierro, D.
datacite.creatorZamorano, Mauricio
datacite.creatorHerrera Belen, Lisandra
datacite.creatorRisopatron, Jennie
datacite.creatorValdebenito, Ivan
datacite.creatorNorambuena, Juan-Alejandro
datacite.creatorFarias, Jorge G.
datacite.date2021
datacite.rightsRegistro bibliográfico
datacite.subjectSalmo salar
datacite.subjectMotility
datacite.subjectIn vitro
datacite.subjectIn silico
datacite.subjectChannel
datacite.titleRole of three plasma membrane Ca2+-binding proteins in the sperm motility of Atlantic salmon (Salmo salar)
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.date.accessioned2025-10-06T14:21:42Z
dc.date.available2025-10-06T14:21:42Z
dc.description.abstractSalmonid sperm motility is regulated by different factors, such as ion concentration, osmotic pressure, pH, and temperature. The Ca2+ cation-dependent regulation and Ca2+-binding proteins are key regulatory mechanisms in the sperm activation process. In this study, the function of transient receptor potential ankyrin 1, transient receptor potential cation channel subfamily V member 4, and Na+/Ca2+ exchanger 1 on the sperm motility of Salmo salar were studied in vitro and in silico. Sperm inhibition assay results showed that the inhibition of the Na+/Ca2+ exchanger produced a significant decrease in total and progressive motility (p < 0.0001). The molecular docking-based analysis showed that inhibitor KB-R7943 binds strongly (-6.0 kcal/mol) in the Ca2+binding region in this exchanger. Additionally, it was demonstrated that, in contrast to Na+/Ca2+ exchange, neither transient receptor potential ankyrin 1 nor transient receptor potential cation channel subfamily V member 4 was directly associated with sperm motility. This research provides new and useful insights into the role of Ca2+-binding proteins present in the plasma membrane that regulate the sperm motility of Salmo salar.
dc.description.ia_keywordsperm, motility, binding, transient, receptor, potential, cation
dc.identifier.issn1873-5622
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6747
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceAquaculture
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2021
oaire.citationTitleAquaculture
oaire.citationVolume538
oaire.fundingReferenceANID FONDECYT 1180387 (Regular)
oaire.fundingReferenceUFRO Beca Doctorado
oaire.licenseConditionCopyright © Elsevier Inc, 2021
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionSCOPUS
uct.indizacionWOS
uct.indizacionDOAJ
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