Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 °C in Cortland medium

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datacite.alternateIdentifier.citationAnimal Reproduction Science, 197, 125-117, 2018
datacite.alternateIdentifier.doi10.1016/j.anireprosci.2018.08.019
datacite.alternateIdentifier.issn0378-4320
datacite.creatorUlloa-Rodriguez, Patricio
datacite.creatorContreras, Pablo
datacite.creatorDumorne, Kelly
datacite.creatorLee-Estevez, Manuel
datacite.creatorDiaz, Rommy
datacite.creatorFigueroa, Elias
datacite.creatorValdebenito, Ivan
datacite.creatorRisopatron, Jennie
datacite.creatorFarias, Jorge G.
datacite.date2018
datacite.rightsRegistro bibliográfico
datacite.subjectShort-term storage
datacite.subjectFish spermatozoa
datacite.subjectSperm function
datacite.subjectAquaculture
datacite.titlePatagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 °C in Cortland medium
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:39Z
dc.date.available2025-10-06T14:21:39Z
dc.description.abstractPatagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The aim of this study is to evaluate the ultrastructure and quality of E. maclovinus sperm during refrigerated storage. To address this objective, scanning electron microscopy (SEM), cytofluorimetric analysis (membrane integrity; reactive oxygen species generation; mitochondrial membrane potential) and cell respiration/mitochondrial-function analysis (ATP content; oxygen consumption) could be useful for optimizing or improving management for artificial reproduction of this species. Severe damage of plasma membranes was observed by SEM at day 7 and 14 of in vitro storage. Analyses of sperm quality were conducted during the 14-day cold storage period when sperm were in diluted (with Cortland solution) and undiluted conditions. When there were diluted conditions, there was greater preservation of motile capacity (from day-7; P < 0.05), membrane integrity (from day-7; P < 0.05), mitochondrial membrane potential (from day-10; P < 0.05) and ATP stores (from day-3; P < 0.05). Oxygen consumption indicators were 18.6% +/- 14.7% greater in the undiluted samples from day-3, and 32.1% +/- 2.1% of the total spermatozoa had ample amounts of superoxide anion in both undiluted and diluted semen on day-0. The use of Cortland solution extended the viability of sperm when there were longer storage times. Factors that have a greater effect on the quality of semen during storage are reactive oxygen species generation and ATP depletion. In conclusion, Patagonian blenny spermatozoa can be stored at 4 degrees C between 7 and 10 days using Cortland solution.
dc.description.ia_keywordstorage, species, sperm, were, quality, membrane, oxygen
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6724
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAnimal Reproduction Science
dc.subject.ia_odsODS 12: Producción y consumo responsables
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2018
oaire.citationEndPage125
oaire.citationStartPage117
oaire.citationTitleAnimal Reproduction Science
oaire.citationVolume197
oaire.fundingReferenceCONICYT Beca Doctorado 21140852
oaire.fundingReferenceCONICYT ANID FONDECYT 1151315, 1180387 (Regular), 3180765 (Postdoctorado)
oaire.licenseConditionCopyright © Elsevier B.V, 2018
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus|PubMed
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