Spermatology and sperm ultrastructure in farmed coho salmon (Oncorhynchus kisutch)

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datacite.alternateIdentifier.citationAquaculture, 547, 2022
datacite.alternateIdentifier.doi10.1016/j.aquaculture.2021.737471
datacite.alternateIdentifier.issn0044-8486
datacite.creatorSandoval-Vargas, Leydy
datacite.creatorRisopatr, Jennie
datacite.creatorDumorne, Kelly
datacite.creatorFarias, Jorge
datacite.creatorFigueroa, Elias
datacite.creatorValdebenito, Ivan
datacite.date2022
datacite.rightsRegistro bibliográfico
datacite.subjectFlow cytometry
datacite.subjectSperm motility
datacite.subjectSpermatology
datacite.subjectScanning electron microscopy
datacite.subjectTEM
datacite.titleSpermatology and sperm ultrastructure in farmed coho salmon (Oncorhynchus kisutch)
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:42Z
dc.date.available2025-10-06T14:21:42Z
dc.description.abstractFish sperm quality assessment is an essential process for commercial production. The goal of this study was to evaluate the quality of fresh milt of farmed coho salmon using a functional and structural approach. A total of 37 males were used to determined parameters such as sperm concentration, pH and osmolality of seminal plasma, viability, mitochondrial membrane potential and fertility. In addition, ultrastructure and morphology were assessed through transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The results show that coho salmon release a large volume of milt (55.08 mL +/- 20.14 mL) with a mean concentration of 13.45 +/- 3.40 x 109 spermatozoa mL-1. A positive correlation was found between sperm concentration and spermatocrit (P < 0.05; r = 0.8447). Milt contained a high percentage of motile sperm (85.00-95.00%) with a mean motility period of 45.25 +/- 2.56 s, viability of 95.24 +/- 2.60% and mitochondrial membrane potential of 91.33 +/- 4.03%. The fertility rate was also high (96.00 +/- 4.16%). SEM and TEM images revealed that mature spermatozoa of coho salmon have a total length of 32.85 +/- 1.25 mu m, are anacrosomal, and consist of an ovoid head, a short cylindrical mid-piece and a long flagellum which can be differentiated into a long main-piece and a short end-piece. The posterior part of the nucleus contains the nuclear fossa in which are located the distal and proximal centrioles, perpendicular to each other. There is only one mitochondrion, positioned as a ring in the mid-piece. The flagellum has two lateral side-fins, apparently of unequal length, and shows the typical 9 + 2 axoneme. These findings indicate that most of milt and sperm traits are within the range reported for other salmonids. Similarly, the results of morphology and ultrastructure are consistent with other salmonids, especially rainbow trout and brown trout.
dc.description.ia_keywordsperm, milt, coho, salmon, piece, concentration, ultrastructure
dc.identifier.issn1873-5622
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6749
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAquaculture
dc.subject.ia_odsODS 14: Vida submarina
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2022
oaire.citationTitleAquaculture
oaire.citationVolume547
oaire.fundingReferenceDoctorado en Ciencias Agropecuarias UCT 4136-2018
oaire.fundingReferenceANID FONDECYT 1180387, 1211246 (Regular), 3180765 (Postdoctorado)
oaire.fundingReferenceUCT VRIP FEQUIP2019-INRN-03
oaire.licenseConditionCopyright © Elsevier Inc, 2022
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionSCOPUS
uct.indizacionWOS
uct.indizacionDOAJ
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