Increased Drought and Salinity Tolerance in Citrus aurantifolia (Mexican Lemon) Plants Overexpressing Arabidopsis CBF3 Gene

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datacite.alternateIdentifier.citationJournal of Soil Science and Plant Nutrition, 20 (1), 252-244, 2020
datacite.alternateIdentifier.doi10.1007/s42729-019-00130-y
datacite.alternateIdentifier.issn0718-9508
datacite.creatorRomero-Romero, Jesús Lucina
datacite.creatorInostroza-Blancheteau, Claudio
datacite.creatorReyes-Díaz, Marjorie M.
datacite.creatorMatte, Juan Pablo
datacite.creatorAquea, Felipe
datacite.creatorEspinoza, Carmen
datacite.creatorGil, Pilar M.
datacite.creatorArce-Johnson, Patricio
datacite.date2020
datacite.rightsRegistro bibliográfico
datacite.subjectAbiotic Stress
datacite.subjectCbf3 Transcription Factor
datacite.subjectDrought Tolerance
datacite.subjectTransgenics Citrus
datacite.titleIncreased Drought and Salinity Tolerance in Citrus aurantifolia (Mexican Lemon) Plants Overexpressing Arabidopsis CBF3 Gene
dc.contributor.authorINOSTROZA BLANCHETEAU, CLAUDIO ANDRES
dc.description.abstractCitrus are a globally important fruit crop. Abiotic stressors such as drought and salinity adversely affect physiological citrus performance and survival. With the aim of improving drought tolerance in citrus plants, we constructed transgenic lines of Citrus lemon overexpressing the Arabidopsis transcription factor CBF3. Molecular, physiological, and quantitative real-time analyses showed high expression of AtCBF3 in three selected transgenic lines. During a 15-day treatment of water deficit by cessation of irrigation, the transgenic lines LM2 and LM14 showed lower stomatal conductance and transpiration paired with lower photosynthesis, whereas transgenic line LM7 maintained its photosynthesis, declining stomatal conductance, and transpiration compared to WT plants, which is manifested into more efficient water use. The genes CsRafS1 and CsGolS1 showed similar or greater expression in one of the transgenic lines with respect to control plants. Moreover, transgenic lines were more tolerant to saline stress and presented a greener phenotype with increased chlorophyll content in leaf discs compared to WT plants. In addition, a lower electrical conductivity in solution was observed in transgenic lines. Furthermore, all transgenic lines exhibited significantly less accumulation of reactive oxygen species than WT plants. Together, these results suggest the potential for heterologous expression of the AtCBF3 gene to mediate tolerance to hydric and saline stress in citrus plants. © 2020 Elsevier B.V., All rights reserved.
dc.description.ia_keywordtransgenic, plants, lines, citrus, drought, tolerance, showed
dc.identifier.issn0718-9516
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3960
dc.language.isoen
dc.publisherSpringer Nature
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceJournal of Soil Science and Plant Nutrition
dc.subject.ia_odsODS 6: Agua limpia y saneamiento
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2020
oaire.citationEndPage252
oaire.citationIssue1
oaire.citationStartPage244
oaire.citationTitleJournal of Soil Science and Plant Nutrition
oaire.citationVolume20
oaire.fundingReferenceCONACYT México SC392465
oaire.fundingReferenceCOTEBAL 1865
oaire.fundingReferenceCONICYT Beca Doctorado Extranjeros 63130094
oaire.fundingReferenceANID FONDECYT 1150220
oaire.fundingReferenceCONICYT ANID PAI 82140040
oaire.fundingReferenceCAPES FB-002-2014
oaire.licenseConditionCopyright © Sociedad Chilena de la Ciencia del Suelo, 2019
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication25287ee1-cae8-401d-b42c-ee3f8fc05d2a
relation.isAuthorOfPublication.latestForDiscovery25287ee1-cae8-401d-b42c-ee3f8fc05d2a
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionCAB Abstracts
uct.indizacionAGRICOLA
uct.indizacionEnvironmental Abstracts
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