Effects of cryopreservation on mitochondria of fish spermatozoa

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datacite.alternateIdentifier.citationReviews in Aquaculture, 9 (1), 87-76, 2017
datacite.alternateIdentifier.doi10.1111/raq.12105
datacite.alternateIdentifier.issn1753-5123
datacite.creatorFigueroa, Elias
datacite.creatorValdebenito, Ivan
datacite.creatorZepeda, Andrea B.
datacite.creatorFigueroa, Carolina A.
datacite.creatorDumorne, Kelly
datacite.creatorCastillo, Rodrigo L.
datacite.creatorFarias, Jorge G.
datacite.date2017
datacite.rightsRegistro bibliográfico
datacite.subjectATP
datacite.subjectcryodamage
datacite.subjectfish spermatozoa
datacite.subjectmitochondrion
datacite.subjectmotility
datacite.titleEffects of cryopreservation on mitochondria of fish spermatozoa
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.description.abstractThe development of sperm cryopreservation has enabled transcendental changes to occur in the reproductive biotechnology of both mammals and fish; it has become a basic tool for animal improvement. Nevertheless, these protocols cause damage to cell structure and physiology, altering sperm functioning due to cryo-injuries during freezing and thawing. However, studies of the effects on the structural, functional and genomic stability of the mitochondria in fish spermatozoa during cryopreservation are still lacking. The object of this review was to analyse the effect of cryopreservation on mitochondrial metabolic pathways in fish spermatozoa. This effect is related with the bioenergy mechanism for flagellar movement during the activation of sperm motility. In teleost fish, the mitochondria may be cylindrical, spherical or irregular in shape and adhere in a helicoidal or conical pattern to the middle piece. The salmonidae have only a single mitochondrion, but this may vary in other species; the mitochondria provide the flagellum with energy during sperm motility, when sperm respiration is essential. The effects of cryopreservation can induce structural damage to the mitochondria, altering the biochemical process involved in ATP production and thus causing a reduction in sperm motility. Fragmentation damage to nuclear DNA and diminution in sperm motility is mainly associated with damage to the structure and metabolic functioning of the mitochondrion. A direct correlation exists between the mitochondrial membrane potential (transmembrane integrity, Delta Psi(m)) and the motility and fertilizing capacity of the cryopreserved spermatozoa, confirming that this organelle is the energy nucleus of the spermatozoon and that the cessation or prolongation of motility and successful fertilization depend on the availability of ATP in the spermatozoa. Further works are need to incorporate biotechnology studies, at cell and molecular level, of the possible effects of cryopreservation on mitochondrial DNA, enzymatic or metabolic modifications of the citric acid cycle, and the oxidative phosphorylation process in the inner membrane, as well as studies of the mitochondrial ultrastructure. Thus, mitochondrial dynamics could be established as a potential target for therapeutic strategies.
dc.description.ia_keywordsperm, cryopreservation, motility, fish, mitochondria, spermatozoa, mitochondrial
dc.identifier.issn1753-5131
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3716
dc.language.isoen
dc.publisherWiley
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceReviews in Aquaculture
dc.subject.ia_odsODS 7: Energía asequible y no contaminante
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2017
oaire.citationEndPage87
oaire.citationIssue1
oaire.citationStartPage76
oaire.citationTitleReviews in Aquaculture
oaire.citationVolume9
oaire.fundingReferenceCONICYT ANID FONDECYT 1151315 (Regular)
oaire.fundingReferenceANID FONDEF D10I1064
oaire.fundingReferenceDIUFRO DI15-2018
oaire.licenseConditionCopyright © Wiley Publishing Asia Pty Ltd, 2015
oaire.resourceTypeArtículo de revisión
oaire.resourceType.enReview
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionAquatic Sciences & Fisheries Abstracts
uct.indizacionCAB Abstracts
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