Effects of cryopreservation on mitochondrial function and sperm quality in fish

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datacite.alternateIdentifier.citationAquaculture, 511, 2019
datacite.alternateIdentifier.doi10.1016/j.aquaculture.2019.06.004
datacite.alternateIdentifier.issn0044-8486
datacite.creatorVillalobos, Elías Figueroa
datacite.creatorLee-Estevez, Manuel
datacite.creatorValdebenito, Iván
datacite.creatorWatanabe, Ii Sei
datacite.creatorPinheiro de Souza Oliveira, Ricardo Pinheiro S.
datacite.creatorRomero, Jaime
datacite.creatorCastillo, Rodrigo L.
datacite.creatorFarias, Jorge G.
datacite.date2019
datacite.rightsRegistro bibliográfico
datacite.subjectAntioxidant Defences
datacite.subjectCryopreservation
datacite.subjectFish Spermatozoa
datacite.subjectMitochondrial Function
datacite.subjectSalmo Salar
datacite.subjectSperm Ultrastructure
datacite.subjectAntioxidant
datacite.subjectCryopreservation
datacite.subjectMitochondrial Dna
datacite.subjectSalmonid
datacite.subjectSperm
datacite.subjectUltrastructure
datacite.subjectAnimalia
datacite.subjectSalmo Salar
datacite.titleEffects of cryopreservation on mitochondrial function and sperm quality in fish
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:42Z
dc.date.available2025-10-06T14:21:42Z
dc.description.abstractThe development of cryopreservation techniques has led to important changes in animal reproductive biotechnology. However, these techniques are associated with cellular and molecular damage, affecting the mitochondrial function and quality of spermatozoa; moreover studies in fish are limited. In this work, the effects of cryopreservation on ultrastructure, mitochondrial function and antioxidant defences in Atlantic salmon (Salmo salar) spermatozoa were assessed, along with intracellular calcium (Ca2+)<inf>i</inf>, mitochondrial DNA sequence and sperm function (motility and fertilization rate). Significant ultrastructure alterations of the middle piece and mitochondria were observed in cryopreserved spermatozoa as compared to controls. Oxygen consumption and ATP were also significantly different in cryopreserved spermatozoa, and in spermatozoa incubated with electron transport chain (ETC) uncouplers and inhibitors. Mitochondrial membrane potential, motility, fertilization rate and Ca2+ <inf>i</inf> in cryopreserved spermatozoa displayed significant reductions compared to fresh spermatozoa. Mitochondrial potential correlated significantly with motility and fertilization rate. A redox imbalance was observed in frozen spermatozoa due to increased lipid peroxidation and superoxide anion production as compared to fresh spermatozoa. Likewise, increased activity of glutathione peroxidase and total glutathione (GSH/GSSG), as well as reduced catalase activity, were observed in comparison with fresh spermatozoa. Our results contribute to a better understanding of cryodamage to mitochondrial functions in fish spermatozoa, and enabled us to establish potential quality assessment indicators. The data suggest that cryopreservation induces a reduction in overall sperm quality and functionality through disruption of the mitochondrial ultrastructure and function, leading to energy depletion and increased oxidative stress. This knowledge may also lead to the identification of a potential biotechnological tool for improving reproductive efficiency in species of commercial and biological interest. © 2019 Elsevier B.V., All rights reserved.
dc.description.ia_keywordspermatozoa, mitochondrial, function, cryopreservation, quality, were, potential
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6757
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAquaculture
dc.subject.ia_odsODS 7: Energía asequible y no contaminante
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2019
oaire.citationTitleAquaculture
oaire.citationVolume511
oaire.fundingReferenceCONICYT ANID FONDECYT 3180765 (Postdoctorado)
oaire.fundingReferenceANID FONDECYT 1180387, 1171129 (Regular)
oaire.fundingReferenceCONICYT Beca Doctorado 21150246
oaire.licenseConditionCopyright © Elsevier Inc, 2019
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
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relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Biológicas y Químicas
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionSCOPUS
uct.indizacionWOS
uct.indizacionDOAJ
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