Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)

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datacite.alternateIdentifier.citationZygote, 26 (6), 448-443, 2018
datacite.alternateIdentifier.doi10.1017/S0967199418000217
datacite.alternateIdentifier.issn1469-8730
datacite.creatorValdebenito, Iván
datacite.creatorVillalobos, Elías Figueroa
datacite.creatorSandoval-Vargas, Leydy Y.
datacite.creatorContreras-Mellado, Pablo
datacite.creatorSánchez-Caamaño, Juan Carlos
datacite.creatorFarias, Jorge G.
datacite.creatorRisopatron, Jennie
datacite.date2018
datacite.rightsRegistro bibliográfico
datacite.subjectCleavage
datacite.subjectEleginops Maclovinus
datacite.subjectKeywordsblastomeres
datacite.subjectNotothenid
datacite.subjectSegmentation
datacite.subjectAnimal
datacite.subjectBlastoma
datacite.subjectCytology
datacite.subjectEmbryology
datacite.subjectFemale
datacite.subjectMale
datacite.subjectNonmammalian Embryo
datacite.subjectPerciformes
datacite.subjectPhysiology
datacite.subjectAnimals
datacite.subjectBlastomeres
datacite.subjectEmbryo, Nonmammalian
datacite.subjectFemale
datacite.subjectMale
datacite.titleCharacterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:22:11Z
dc.date.available2025-10-06T14:22:11Z
dc.description.abstractThere is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically. Of a 'pool' of incubated eggs at 10.7 ± 0.5°C, 100 microphotographs of blastodiscs were extracted at different incubation periods from 0.25 to 5 h after fertilization and analyzed. Blastodiscs taken at 3.5, 4.0 and 5.0 h were characterized and classified into symmetric or asymmetric groups according to their morphology. The proportions of length (L) and width (W) of each blastomere were determined to establish its symmetry. Additionally, 20 microphotographs of blastodiscs of normal appearance were analyzed morphologically (control blastodisc: CB) and compared other blastodiscs (4.0 and 5.0 h). The results showed that before fertilization oocytes presented a somehow turgid aspect (maximum average diameter of 987 ± 41 Am) and after fertilization and hydration, their diameter increased to 1001.5 ± 11 Am (but not statistically significant) and presented a spherical shape. First cleavage ends after 3.5 h of development, forming two blastomeres 467 ± 45 ?m length (L) and 328 ± 21 ?m width (W) with a L/W ratio of 1.43 ± 0.19. The second cleavage ends after development at 4.5 h forming four blastomeres 238 ± 65 ?m length and 227 ± 65 ?m width with a ratio L/W of 1.06 ± 0.09. Five categories were identified during the blastomere characterization: 70% normal or symmetric; 8% with odd numbers of blastomeres; 6% unequal; 6% 'pie shaped' and 10% amorphous. © 2019 Elsevier B.V., All rights reserved.
dc.description.ia_keywordblastomeres, were, blastodiscs, after, cleavage, maclovinus, first
dc.identifier.issn0967-1994
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6972
dc.language.isoen
dc.publisherCambridge University Press
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceZygote
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2018
oaire.citationEndPage448
oaire.citationIssue6
oaire.citationStartPage443
oaire.citationTitleZygote
oaire.citationVolume26
oaire.fundingReferenceFundación Chile Estación Experimental Quillaipe
oaire.fundingReferenceANID FONDECYT 1151315, 1180387 (Regular)
oaire.fundingReferenceCONICYT MEC 80140066
oaire.licenseConditionCopyright © Cambridge University Press 2018
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionMEDLINE
uct.indizacionEMBASE
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