Flow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators

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datacite.alternateIdentifier.citationApplied Microbiology and Biotechnology, 107 (45718), 590-581, 2023
datacite.alternateIdentifier.doi10.1007/s00253-022-12318-x
datacite.alternateIdentifier.issn0175-7598
datacite.creatorGonzález, Karina
datacite.creatorSalinas, Alejandro
datacite.creatorPinto-Ibieta, Fernanda
datacite.creatorNavia, Rodrigo
datacite.creatorLiu, Shijie
datacite.creatorCea, Mara
datacite.date2023
datacite.rightsRegistro bibliográfico
datacite.subjectBioreactors
datacite.subjectFlow Cytometry
datacite.subjectMixed Microbial Cultures
datacite.subjectPolyhydroxyalkanoates
datacite.subjectCarbon
datacite.subjectPolyhydroxyalkanoates
datacite.subjectPolyhydroxybutyrate
datacite.subjectBatch Reactors
datacite.subjectFlow Cytometry
datacite.subjectFood Supply
datacite.subjectMetabolism
datacite.subjectAttractive Strategies
datacite.subjectMetabolic Activity
datacite.subjectMetabolic State
datacite.subjectMixed Microbial Culture
datacite.subjectPhysiological State
datacite.subjectPolyhydroxyalkanoates
datacite.subjectPolyhydroxybutyrate
datacite.subjectProcess Tools
datacite.subjectProduction Process
datacite.subjectSequencing Batch Reactors
datacite.subjectMicroorganisms
datacite.subjectCarbon
datacite.subjectDissolved Oxygen
datacite.subjectPolyhydroxyalkanoic Acid
datacite.subjectPolyhydroxybutyrate
datacite.subjectAccumulation
datacite.subjectBioreactor
datacite.subjectFamine
datacite.subjectFlow Cytometry
datacite.subjectMicrobial Activity
datacite.subjectAnalytic Method
datacite.subjectArticle
datacite.subjectBacterial Strain
datacite.subjectCarbon Source
datacite.subjectHydraulic Retention Time
datacite.subjectMetabolism
datacite.subjectMicroorganism
datacite.subjectNonhuman
datacite.subjectOperation Duration
datacite.subjectSolids Retention Time
datacite.subjectWaste Water Treatment Plant
datacite.subjectFermentation
datacite.subjectBioreactors
datacite.subjectFermentation
datacite.subjectFlow Cytometry
datacite.titleFlow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators
dc.contributor.authorPINTO IBIETA, FERNANDA EMILIA
dc.date.accessioned2025-10-06T14:21:41Z
dc.date.available2025-10-06T14:21:41Z
dc.description.abstractAbstract: The use of mixed microbial cultures (MMCs) is seen as an attractive strategy for polyhydroxyalkanoate (PHA) production. In order to optimize the MMC-PHA production process, tools are required to improve our understanding of the physiological state of the PHA-storing microorganisms within the MMC. In the present study, we explored the use of flow cytometry to analyse the metabolic state and polyhydroxybutyrate (PHB) content of the microorganisms from an MMC-PHA production process. A sequencing batch reactor under a feast and famine regime was used to enrich an MMC with PHB-storing microorganisms. Interestingly, once the PHB-storing microorganisms are selected, the level of PHB accumulation depends largely on the metabolic state of these microorganisms and not exclusively on the consortium composition. These results demonstrate that flow cytometry is a powerful tool to help to understand the PHA storage response of an MMC-PHA production process. Key points: Flow cytometry allows to measure PHB content and metabolic activity over time. Microorganisms showing high PHB content also have high metabolic activity. PHB producers with low metabolic activity show low PHB content. © 2023 Elsevier B.V., All rights reserved.
dc.description.ia_keywordmicroorganisms, metabolic, production, flow, cytometry, content, process
dc.identifier.issn1432-0614
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6744
dc.language.isoen
dc.publisherSpringer
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceApplied Microbiology and Biotechnology
dc.subject.ia_oecd1nIngeniería y Tecnología
dc.subject.ia_oecd2nIngeniería Química
dc.subject.ia_oecd3nIngeniería Química
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2023
oaire.citationEndPage590
oaire.citationIssue2-3
oaire.citationStartPage581
oaire.citationTitleApplied Microbiology and Biotechnology
oaire.citationVolume107
oaire.fundingReferenceANID PFCHA Doctorado 21191476
oaire.fundingReferenceANID ANILLOS ACT172128 (GAMBIO)
oaire.fundingReferenceANID FONDECYT 3200748 (Postdoctorado)
oaire.licenseConditionCopyright © Springer Nature, 2023
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication222c7a9b-189a-4a82-8d38-89ca7765bd44
relation.isAuthorOfPublication.latestForDiscovery222c7a9b-189a-4a82-8d38-89ca7765bd44
uct.catalogadorjvu
uct.comunidadIngenieríaen_US
uct.departamentoDepartamento Procesos Industriales
uct.facultadFacultad de Ingeniería
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionScimago
uct.indizacionPubMed
uct.indizacionCrossref
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