High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor
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Date
Authors
Vidal-Seguel, Nicolás
Cabrera, Camilo Daniel
Ferrada, Luciano Esteban
Artigas-Arias, Macarena
Alegría-Molina, Andrea
Sanhueza, Sergio Andrés
Flores, Alejandra
Huard, Nolberto
Sapunar, Jorge
Salazar Navarrete, Luis A.
Cabrera, Camilo Daniel
Ferrada, Luciano Esteban
Artigas-Arias, Macarena
Alegría-Molina, Andrea
Sanhueza, Sergio Andrés
Flores, Alejandra
Huard, Nolberto
Sapunar, Jorge
Salazar Navarrete, Luis A.
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Date
Datos de publicación:
10.1016/j.exger.2023.112280
Keywords
Elderly - Exercise - High-intensity Interval Training - Nets - Neutrophil Extracellular Trap - Adult - Aerobic Capacity - Age Distribution - Aged - Article - Blood Sampling - Body Mass - Cell Culture - Cohort Analysis - Controlled Study - Effect Size - Elderly Care - Exercise - Health Program - High Intensity Interval Training - Human - Human Cell - Human Experiment - Live Cell Imaging - Live Neutrophil Imaging - Male - Men's Health - Netosis - Neutrophil - Neutrophil Extracellular Trap - Normal Human - Serum - Treatment Duration - Aging - Extracellular Trap - Genetic Procedures - Aged - Aging - Biosensing Techniques - Extracellular Traps - High-intensity Interval Training - Humans - Male - Neutrophils
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Abstract
Neutrophil extracellular trap formation (NETosis) is a mechanism used by neutrophils to capture pathogens with their own DNA. However, the exacerbation of this immune response is related to serious inflammatory diseases. Aging is known to lead to an excessive increase in NETosis associated with various diseases. Under this scenario, the search for strategies that regulate the release of NETosis in older people becomes relevant. High-intensity interval training (HIIT) involves repeated bouts of relatively intense exercise with alternating short recovery periods. This training has shown beneficial effects on health parameters during aging and disease. However, little is known about the potential role of HIIT in the regulation of NETosis in healthy older people. The aim of this study was to evaluate the induction of NETosis by serum from healthy young and older men, before and after 12 weeks of HIIT using healthy neutrophils as a biosensor. HIIT was performed 3 times per week for 12 weeks in young (YOUNG; 21 ± 1 years, BMI 26.01 ± 2.64 kg?m?2, n = 10) and older men (OLDER; 66 ± 5 years, BMI 27.43 ± 3.11 kg?m?2, n = 10). Serum samples were taken before and after the HIIT program and NETosis was measured with live cell imaging in donated neutrophils cultured with serum from the participants for 30 h. Our results showed that serum from older men at baseline induced greater baseline NETosis than younger men (p < 0.05; effect size, ?0.8), and 12 weeks of HIIT significantly reduced (Interaction Effect, p < 0.05; effect size, 0.134) the induction of NETosis in older men. In conclusion, HIIT is a feasible non-invasive training strategy modulating NETosis induction. Additionally, the use of neutrophils as a biosensor is an effective method for the quantification of NETosis induction in real time. © 2023 Elsevier B.V., All rights reserved.
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Keywords
Elderly , Exercise , High-intensity Interval Training , Nets , Neutrophil Extracellular Trap , Adult , Aerobic Capacity , Age Distribution , Aged , Article , Blood Sampling , Body Mass , Cell Culture , Cohort Analysis , Controlled Study , Effect Size , Elderly Care , Exercise , Health Program , High Intensity Interval Training , Human , Human Cell , Human Experiment , Live Cell Imaging , Live Neutrophil Imaging , Male , Men's Health , Netosis , Neutrophil , Neutrophil Extracellular Trap , Normal Human , Serum , Treatment Duration , Aging , Extracellular Trap , Genetic Procedures , Aged , Aging , Biosensing Techniques , Extracellular Traps , High-intensity Interval Training , Humans , Male , Neutrophils
Citation
10.1016/j.exger.2023.112280