Morphometric of blastomeres in Salmo salar

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datacite.alternateIdentifier.citationZygote, 22 (4), 475-470, 2014
datacite.alternateIdentifier.doi10.1017/S0967199412000718
datacite.alternateIdentifier.issn0967-1994
datacite.creatorEffer, Brian R.
datacite.creatorSanchez, Ruben R.
datacite.creatorUbilla, Andrea M.
datacite.creatorFigueroa, Elias V.
datacite.creatorValdebenito, Ivan I.
datacite.date2014
datacite.rightsRegistro bibliográfico
datacite.subjectBlastomeres
datacite.subjectEmbryos
datacite.subjectMorphometric
datacite.subjectSalmo salar
datacite.titleMorphometric of blastomeres in Salmo salar
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.description.abstractFor Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the first blastomeres of S. salar. From a pool of eggs incubated at 7.5 degrees C, 100 microphotographs of blastodiscs were extracted and analyzed at different incubation periods: 12, 14, 16, 20 or 24 h. Blastodiscs were characterized morphologically after 16, 20 or 24 h incubation, and classified into symmetric or asymmetric groups according to their morphology. The ratio of length (L) versus width (W) of each blastomere was determined, to establish its symmetry. In addition, 20 microphotographs of blastodiscs of normal appearance were analysed morphologically (control blastodisc: CB) for comparison (20 or 24 h). Results show that the first cleavage ends after 16 h of development. Seven categories were established during blastomere characterization: 47% normal (G1); 27% with dispersed margins (G2); 10% unequal (G3); 9% 'pie-shaped' (G4); 3% amorphous (G5); 2% three equal blastomeres and one different one (G6); and 2% with eccentric cleavage (G7). Although the incidence of abnormal cleavage in S. salar is uncertain, there is a potential for some asymmetries to be corrected during embryogenesis to generate viable individuals. More studies are necessary to correlate these abnormal cleavage patterns with indicators of quality in the later stages of embryogenesis in this species, to establish a quality assessment tool for gametes and/or embryos in salmonid species.
dc.description.ia_keywordsalar, first, blastomeres, were, cleavage, during, development
dc.identifier.issn1469-8730
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/2649
dc.language.isoen
dc.publisherCambridge University Press
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceZygote
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nEcología
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2014
oaire.citationEndPage475
oaire.citationIssue4
oaire.citationStartPage470
oaire.citationTitleZygote
oaire.citationVolume22
oaire.fundingReferenceANID FONDECYT 1120705 (Regular)
oaire.licenseConditionCopyright © Cambridge University Press, 2013
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionMEDLINE
uct.indizacionEMBASE
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