Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

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datacite.alternateIdentifier.citationJournal of Fish Biology, 89 (3), 1550-1537, 2016
datacite.alternateIdentifier.doi10.1111/jfb.13052
datacite.alternateIdentifier.issn0022-1112
datacite.creatorFigueroa, E.
datacite.creatorValdebenito, I.
datacite.creatorMerino, O.
datacite.creatorUbilla, A.
datacite.creatorRisopatron, J.
datacite.creatorFarias, J. G.
datacite.date2016
datacite.rightsRegistro bibliográfico
datacite.subjectfertility
datacite.subjectfish
datacite.subjectsperm function
datacite.subjectspermatozoa freezing
datacite.titleCryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.description.abstractThe objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as the treatment (T) and fresh semen as the control (F). Straws of 05ml of sperm suspension were frozen in 4cm of N2L. They were thawed in a thermoregulated bath (40 degrees C). After thawing, the percentage of spermatozoa with fragmented DNA [transferase dUTP (deoxyuridine triphosphate) nick-end labelling (TUNEL)], plasma membrane integrity (SYBR-14/PI) and mitochondrial membrane potential (MMit, JC-1) were evaluated by flow cytometry and motility was evaluated by optical microscope under stroboscopic light. The fertilization rates of the control and treatment semen were tested at a sperm density of 15x10(7) spermatozoa oocyte(-1), by observation of the first cleavages after 16h incubation at 10 degrees C. In the cryopreserved semen (T), the mean +/- s.d. DNA fragmentation was 48 +/- 25%; plasma membrane integrity 752 +/- 63%; mitochondrial membrane potential 517 +/- 36%; motility 585 +/- 53%; curved line velocity (V-CL) 612 +/- 174 mu ms(-1); average-path velocity (V-AP) 501 +/- 173 mu ms(-1); straight-line velocity (V-SL) 591 +/- 184 mu ms(-1); fertilization rate 816 +/- 19%. There were significant differences in the plasma membrane integrity, mitochondrial membrane potential, motility, fertilization rate, V-CL, V-AP and V-SL compared with the controls (P<005). Also the mitochondrial membrane potential correlated with motility, fertilization rate, V-CL and V-SL (r=075; r=059; r=077 and r=079, respectively; P<005); and the fertilization rate correlated with V-CL and V-SL (r=059 and r=055, respectively).
dc.description.ia_keywordmembrane, semen, were, fertilization, sperm, mitochondrial, potential
dc.identifier.issn1095-8649
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/2909
dc.language.isoen
dc.publisherWiley
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceJournal of Fish Biology
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2016
oaire.citationEndPage1550
oaire.citationIssue3
oaire.citationStartPage1537
oaire.citationTitleJournal of Fish Biology
oaire.citationVolume89
oaire.fundingReferenceANID FONDECYT 1151315 (Regular)
oaire.fundingReferenceCONICYT MEC 80140066
oaire.fundingReferenceANID FONDEF D10I1064
oaire.fundingReferenceDIUFRO DI15-2018
oaire.licenseConditionCopyright © The Fisheries Society of the British Isles, 2016
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
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relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionZoological Record
uct.indizacionAquatic Sciences & Fisheries Abstracts
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