Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar)

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datacite.alternateIdentifier.citationAnimal Nutrition, 10, 279-261, 2022
datacite.alternateIdentifier.doi10.1016/j.aninu.2022.04.003
datacite.alternateIdentifier.issn2405-6383
datacite.creatorVillasante, Alejandro
datacite.creatorRodríguez, Héctor
datacite.creatorDantagnan, Patricio P.
datacite.creatorHernández Arias, Adrián J.
datacite.creatorVillalobos, Elías Figueroa
datacite.creatorRomero, Jaime
datacite.date2022
datacite.rightsAcceso abierto
datacite.subjectMicrobiota
datacite.subjectMicrobioma
datacite.subjectSalmón Atlántico
datacite.subjectEsteatosis
datacite.subjectBacterias Lácticas
datacite.subjectProbióticos
datacite.subject.englishAtlantic Salmon
datacite.subject.englishLactic Acid Bacteria
datacite.subject.englishMicrobiome
datacite.subject.englishMicrobiota
datacite.subject.englishProbiotics
datacite.subject.englishSteatosis
datacite.subject.englishC Reactive Protein
datacite.subject.englishFatty Acid Synthase
datacite.subject.englishC Reactive Protein
datacite.subject.englishComplementary Dna
datacite.subject.englishDouble Stranded Dna
datacite.subject.englishEndoplasmic Reticulum Chaperone Bip
datacite.subject.englishFatty Acid Synthase
datacite.subject.englishRna 16s
datacite.subject.englishTranscription Factor Fkhrl1
datacite.subject.englishAmplicon
datacite.subject.englishAnaerosalibacter
datacite.subject.englishAnimal Experiment
datacite.subject.englishAnimal Tissue
datacite.subject.englishArticle
datacite.subject.englishAtopostipes
datacite.subject.englishBacillus
datacite.subject.englishBacterium
datacite.subject.englishBlastopirellula
datacite.subject.englishBody Weight Gain
datacite.subject.englishCarbohydrate Diet
datacite.subject.englishCarbohydrate To Protein Ratio
datacite.subject.englishCell Vacuole
datacite.subject.englishClostridium
datacite.subject.englishClostridium Sensu Stricto
datacite.subject.englishClostridium Xi
datacite.subject.englishControlled Study
datacite.subject.englishCorrelation Analysis
datacite.subject.englishCrp Gene
datacite.subject.englishD6fad Gene
datacite.subject.englishDiet Composition
datacite.subject.englishDna Extraction
datacite.subject.englishDna Sequencing
datacite.subject.englishDna Synthesis
datacite.subject.englishFas Gene
datacite.subject.englishFatty Liver
datacite.subject.englishFeed Efficiency
datacite.subject.englishFoxo3 Gene
datacite.subject.englishFusobacterium
datacite.subject.englishGene
datacite.subject.englishGene Expression
datacite.subject.englishGeobacillus
datacite.subject.englishGrowth Rate
datacite.subject.englishGrp78 Gene
datacite.subject.englishHaematobacter
datacite.subject.englishHigh Throughput Sequencing
datacite.subject.englishHistology
datacite.subject.englishIntestine Flora
datacite.subject.englishLactobacillus
datacite.subject.englishLegionella
datacite.subject.englishLentibacillus
datacite.subject.englishLeuconostoc
datacite.subject.englishLipogenesis
datacite.subject.englishLiver Function
datacite.subject.englishMacrococcus
datacite.subject.englishMarinococcus
datacite.subject.englishNeochlamydia
datacite.subject.englishNocardioides
datacite.subject.englishNonhuman
datacite.subject.englishNutritional Parameters
datacite.subject.englishOceanobacillus
datacite.subject.englishPaenibacillus
datacite.subject.englishPhotobacterium
datacite.subject.englishProtein Diet
datacite.subject.englishPseudoclavibacter
datacite.subject.englishRalstonia
datacite.subject.englishRna Extraction
datacite.subject.englishSalmo Salar
datacite.subject.englishSequence Alignment
datacite.subject.englishSingulisphaera
datacite.subject.englishSpecies Diversity
datacite.subject.englishSpecies Richness
datacite.subject.englishSporosarcina
datacite.subject.englishStreptococcus
datacite.subject.englishTepidimicrobium
datacite.subject.englishWeisella
datacite.titleDietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar)
dc.contributor.authorDANTAGNAN DANTAGNAN, HERMAN PATRICIO
dc.contributor.authorHERNANDEZ ARIAS, ADRIAN JESUS
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.date.accessioned2025-10-06T14:21:39Z
dc.date.available2025-10-06T14:21:39Z
dc.description.abstractAtlantic salmon (Salmo salar) fed a carbohydrate-rich diet exhibit suboptimal growth performance, along with other metabolic disturbances. It is well known that gut microbes play a pivotal role in influencing metabolism of the host, and these microbes can be modified by the diet. The main goal of the present study was to determine the effect of feeding graded levels of digestible carbohydrates to Atlantic salmon on the distal intestine digesta microbiota at 3 sampling times (i.e., weeks 4, 8 and 12), during a 12-week trial. A low carbohydrate-to-high protein diet (LC/HP, 0% wheat starch), a medium carbohydrate-to-medium protein diet (MC/MP, 15% wheat starch) or a high carbohydrate-to-low protein diet (HC/LP, 30% wheat starch) was fed to triplicate fish tanks (27 to 28 fish per tank). We performed an in-depth characterization of the distal intestine digesta microbiota. Further, growth parameters, liver histology and the expression of genes involved in hepatic neolipogenesis in fish were measured. Fish fed a HC/LP diet showed greater hepatosomatic and viscerosomatic indexes (P = 0.026 and P = 0.018, respectively), lower final weight (P = 0.005), weight gain (P = 0.003), feed efficiency (P = 0.033) and growth rate (P = 0.003) compared with fish fed the LC/HP diet. Further, feeding salmon a high digestible carbohydrate diet caused greater lipid vacuolization, steatosis index (P = 0.007) and expression of fatty acid synthase (fas) and delta-6 fatty acyl desaturase (d6fad) (P = 0.001 and P = 0.001, respectively) in the liver compared with fish fed the LC/HP diet. Although, the major impact of feeding a carbohydrate-rich diet to Atlantic salmon in beta diversity of distal intestine digesta microbiota was observed at week 4 (HC/LP vs MC/MP and HC/LP vs LC/HP; P = 0.007 and P = 0.008, respectively) and week 8 (HC/LP vs MC/MP; P = 0.04), no differences between experimental groups were detected after 12 weeks of feeding. Finally, at the end of the trial, there was a negative correlation between lactic acid bacteria (LAB) members, including Leuconostoc and Lactobacillus, with hepatic steatosis level, the hepatosomatic and viscerosomatic indexes as well as the expression of fas and d6fad. Weissella showed negative correlation with hepatic steatosis level and the hepatosomatic index. Finally, further research to explore the potential use of LAB as probiotics to improve liver health in carnivorous fish fed fatty liver-induced diet is warranted. © 2022 Elsevier B.V., All rights reserved.
dc.description.ia_keyworddiet, carbohydrate, fish, salmon, atlantic, growth, feeding
dc.formatPDF
dc.identifier.issn2405-6545
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6719
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAnimal Nutrition
dc.subject.ia_odsODS 3: Salud y bienestar
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2022
oaire.citationEndPage279
oaire.citationStartPage261
oaire.citationTitleAnimal Nutrition
oaire.citationVolume10
oaire.fundingReferenceANID FONDECYT 1200523, 1171129 (Regular), 3160835 (Postdoctorado)
oaire.licenseConditionObra bajo licencia Creative Commons Atribución-No Comercial-Sin Derivadas 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9ed02e88-c517-44ff-b002-91dfa5b40b99
relation.isAuthorOfPublicationd6879a45-cf2a-418d-8f5e-0773cf35f4dd
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uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.nucleosNúcleo de Investigación en Producción Alimentaria
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