Characterization of anti-soybean agglutinin (SBA) IgY antibodies: a new strategy for neutralization of the detrimental biological activity of SBA

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datacite.alternateIdentifier.citationFrontiers in Veterinary Science, Vol. 11, 2024
datacite.alternateIdentifier.doi10.3389/fvets.2024.1382510
datacite.creatorRuiz Diaz, Nancy
datacite.creatorCisternas, Carlos
datacite.creatorSilva, Mauricio
datacite.creatorHernandez, Adrian
datacite.creatorChacana, Pablo
datacite.date2024
datacite.rightsAcceso Abierto
datacite.subjectSoja
datacite.subjectAglutinina
datacite.subjectIgY
datacite.subjectHemaglutinación
datacite.subjectFactor antinutricional
datacite.subject.englishsoybean
datacite.subject.englishagglutinin
datacite.subject.englishIgY
datacite.subject.englishhaemagglutination
datacite.subject.englishantinutritional factor
datacite.titleCharacterization of anti-soybean agglutinin (SBA) IgY antibodies: a new strategy for neutralization of the detrimental biological activity of SBA
dc.date.accessioned27-05-2024 14:27
dc.date.available27-05-2024 14:27
dc.description.abstractAnti-soybean agglutinin (SBA) IgY was produced, and its potential to neutralize the haemagglutinating activity of SBA in vitro was tested. Thirty-five-week-old hens [treatment (n = 5) and control (n = 5)] were immunized with SBA or injected with saline 4 times every 15 days. Eggs were collected after the last immunization, and IgY was extracted using the polyethylene glycol (PEG) method. Serum anti-SBA IgY titres in immunized hens increased after the first immunization and reached a plateau between days 45 and 60. In contrast, specific IgY titres in the control group remained at basal levels throughout the evaluation. Average IgY titres were significantly higher in the treatment group on days 15, 30, 45, and 60. Total IgY content in the egg yolk extract was 38.7 +/- 1.6 and 37.7 +/- 1.5 mg/ml for the treatment and control groups, respectively. The specific anti-SBA IgY titer detected in the egg yolk extract was significantly higher (p < 0.001) for hens in the treatment group compared to the control group, with OD450nm values of 0.98 +/- 0.05 and 0.058 +/- 0.02, respectively. The specificity of anti-SBA IgY was confirmed by the Western blotting, and the inhibition of SBA-induced haemagglutination in vitro was compared with D-galactose, a known molecule that binds to SBA and blocks its binding to erythrocytes. The inhibition of SBA-induced haemagglutination by the anti-SBA IgY reached 512 units of haemagglutination inhibition (UHI), compared to 8 or 256 UHI, respectively, when IgY from control chickens or D-galactose was used. Thus, anti-SBA IgY antibodies were efficiently produced in large quantities and effectively inhibited SBA-induced haemagglutination in vitro.
dc.formatPDF
dc.identifier.issn2297-1769
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5742
dc.language.isoen
dc.publisherFrontiers media S.A.
dc.rightsLicencia Creative Commons Atribución 4.0
dc.sourceFrontiers in Veterinary Science
dspace.entity.typePublication
oaire.citationEdition2024
oaire.citationTitleFrontiers in Veterinary Science
oaire.citationVolume11
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionPubMed
uct.indizacionPMC
uct.indizacionSCOPUS
uct.indizacionDOAJ
uct.indizacionWeb of Science
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