Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))

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datacite.alternateIdentifier.citationAquaculture Reports, 11, 46-42, 2018
datacite.alternateIdentifier.doi10.1016/j.aqrep.2018.05.002
datacite.alternateIdentifier.issn2352-5134
datacite.creatorDumorné, Kelly
datacite.creatorValdebenito, Iván
datacite.creatorContreras, Pablo
datacite.creatorRodríguez, Patricio Ulloa
datacite.creatorRisopatron, Jennie
datacite.creatorVillalobos, Elías Figueroa
datacite.creatorEstevez, Manuel Lee
datacite.creatorDíaz, Rommy
datacite.creatorFarias, Jorge G.
datacite.date2018
datacite.rightsRegistro bibliográfico
datacite.subjectGenypterus Blacodes
datacite.subjectOsmolality
datacite.subjectPh
datacite.subjectSperm Motility
datacite.subjectTemperature
datacite.titleEffect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:42Z
dc.date.available2025-10-06T14:21:42Z
dc.description.abstractIn this research we evaluated simple aspects of the sperm biology of Genypterus blacodes, in particular assessing the effects of pH (6, 7, 8 and 9), temperature (4, 8 and 16 °C) and osmolalities 100% sea water (1010 mOsm/kg, Control), 75% sea water (774 mOsm/kg, T<inf>1</inf>), 50% sea water (488 mOsm/kg, T<inf>2</inf>) and distilled water (0 mOsm/kg, T<inf>3</inf>)) on the motility of Genypterus blacodes intratesticular spermatozoa. In addition, we determined the fertilization rate. Our results show that G. blacodes spermatozoa have a sperm density of 5.35 ± 0.16 × 109 spermatozoa/mL. Sperm motility is initiated on contact with a hyperosmotic swimming medium under normal conditions (1010 mOsm/kg, pH 8 and 8 °C). The longest motility duration (432.48 ± 8.89 s) was recorded at 4 °C. The maximum percentage of motile cells was recorded at 8 °C (65.66 ± 4.95) at osmolality 1010 mOsm/kg, whereas an optimum was observed at pH 8. The fertility rate was 73.9 ± 17%. This is the first report on sperm motility of G. blacodes spermatozoa. In conclusion, the results of this study permit a baseline to be established for further research and protocols for artificial reproduction of this species to be developed and optimized. In addition, the information gathered in this research will be useful for developing the biotechnology of Genypterus blacodes. © 2018 Elsevier B.V., All rights reserved.
dc.description.ia_keywordblacodes, mosm, sperm, motility, genypterus, water, spermatozoa
dc.formatPDF
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6761
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAquaculture Reports
dc.subject.ia_odsODS 6: Agua limpia y saneamiento
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2018
oaire.citationEndPage46
oaire.citationStartPage42
oaire.citationTitleAquaculture Reports
oaire.citationVolume11
oaire.fundingReferenceANID FONDECYT 1151315 (Regular)
oaire.fundingReferenceUniversidad de La Frontera (PhD scholarships)
oaire.licenseConditionObra bajo licencia Creative Commons Atribución-No Comercial-Sin Derivadas 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionSCOPUS
uct.indizacionWOS
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