Molecular characterisation of a calmodulin gene, VcCaM1, that is differentially expressed under aluminium stress in highbush blueberry

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datacite.alternateIdentifier.citationPlant Biology, 15 (6), 1018-1013, 2013
datacite.alternateIdentifier.doi10.1111/j.1438-8677.2012.00722.x
datacite.alternateIdentifier.issn1435-8603
datacite.creatorInostroza-Blancheteau, C.
datacite.creatorAquea, F.
datacite.creatorLoyola, R.
datacite.creatorSlovin, J.
datacite.creatorJosway, S.
datacite.creatorRengel, Z.
datacite.creatorReyes-Diaz, M.
datacite.creatorAlberdi, M.
datacite.creatorArce-Johnson, P.
datacite.date2013
datacite.rightsRegistro bibliográfico
datacite.subjectAbiotic stress
datacite.subjectcalcium
datacite.subjectgene expression
datacite.subjectqRT-PCR
datacite.subjectRACE
datacite.subjectVaccinium corymbosum
datacite.titleMolecular characterisation of a calmodulin gene, VcCaM1, that is differentially expressed under aluminium stress in highbush blueberry
dc.contributor.authorINOSTROZA BLANCHETEAU, CLAUDIO ANDRES
dc.description.abstractCalmodulin (CaM), a small acidic protein, is one of the best characterised Ca2+ sensors in eukaryotes. This Ca2+-regulated protein plays a critical role in decoding and transducing environmental stress signals by activating specific targets. Many environmental stresses elicit changes in intracellular Ca2+ activity that could initiate adaptive responses under adverse conditions. We report the first molecular cloning and characterisation of a calmodulin gene, VcCaM1 (Vaccinium corymbosum Calmodulin 1), in the woody shrub, highbush blueberry. VcCaM1 was first identified as VCAL19, a gene induced by aluminium stress in V.corymbosum L. A full-length cDNA of VcCaM1 containing a 766-bp open reading frame (ORF) encoding 149 amino acids was cloned from root RNA. The sequence encodes four Ca2+-binding motifs (EF-hands) and shows high similarity (99%) with the isoform CaM 201 of Daucus carota. Expression analyses showed that following Al treatment, VcCaM1 message level decreased in roots of Brigitta, an Al-resistant cultivar, and after 48h, was lower than in Bluegold, an Al-sensitive cultivar. VcCAM1 message also decreased in leaves of both cultivars within 2h of treatment. Message levels in leaves then increased by 24h to control levels in Brigitta, but not in Bluegold, but then decreased again by 48h. In conclusion, VcCaM1 does not appear to be directly involved in Al resistance, but may be involved in improved plant performance under Al toxicity conditions through regulation of Ca2+ homeostasis and antioxidant systems in leaves.
dc.description.ia_keywordvccam, calmodulin, stress, under, gene, message, decreased
dc.identifier.issn1438-8677
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3947
dc.language.isoen
dc.publisherWiley
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourcePlant Biology
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2013
oaire.citationEndPage1018
oaire.citationIssue6
oaire.citationStartPage1013
oaire.citationTitlePlant Biology
oaire.citationVolume15
oaire.fundingReferenceFruit Consortium 07Genoma01
oaire.fundingReferenceANID Núcleo Milenio P06-009-F (Plant Functional Genomics)
oaire.fundingReferenceANID FONDECYT 11080231, 1080372 (Regular)
oaire.licenseConditionCopyright © German Botanical Society and The Royal Botanical Society of the Netherlands, 2013
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication25287ee1-cae8-401d-b42c-ee3f8fc05d2a
relation.isAuthorOfPublication.latestForDiscovery25287ee1-cae8-401d-b42c-ee3f8fc05d2a
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionCAB Abstracts
uct.indizacionAGRICOLA
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