Oxidative Stress Induces Changes in Molecular Markers Associated with Ferroptosis in Human Spermatozoa

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datacite.alternateIdentifier.citationWorld Journal of Men'S Health, 42, 2024
datacite.alternateIdentifier.doi10.5534/wjmh.240085
datacite.alternateIdentifier.issn2287-4208
datacite.creatorContreras-Mellado, Pablo
datacite.creatorBravo, Anita
datacite.creatorZambrano, Fabiola
datacite.creatorSánchez G., Raúl Segundo
datacite.creatorBoguen, R.
datacite.creatorRisopatron, Jennie
datacite.creatorMerino, Osvaldo
datacite.creatorUribe, Pamela
datacite.date2024
datacite.rightsAcceso abierto
datacite.subjectArachidonic Acid
datacite.subjectFerroptosis
datacite.subjectOxidative Stress
datacite.subjectSperm Function
datacite.subjectSpermatozoa
datacite.subjectArachidonic Acid
datacite.subjectPhospholipid Hydroperoxide Glutathione Peroxidase
datacite.subjectArachidonic Acid
datacite.subjectMolecular Marker
datacite.subjectPhospholipid Hydroperoxide Glutathione Peroxidase
datacite.subjectReactive Oxygen Metabolite
datacite.subjectArticle
datacite.subjectCell Death
datacite.subjectCell Viability
datacite.subjectConfocal Laser Scanning Microscopy
datacite.subjectControlled Study
datacite.subjectFerroptosis
datacite.subjectFlow Cytometry
datacite.subjectHuman
datacite.subjectHuman Cell
datacite.subjectLipid Peroxidation
datacite.subjectMale
datacite.subjectMitochondrial Membrane Potential
datacite.subjectOxidative Stress
datacite.subjectSperm Donor
datacite.subjectSperm Function
datacite.subjectSperm Quality
datacite.titleOxidative Stress Induces Changes in Molecular Markers Associated with Ferroptosis in Human Spermatozoa
dc.contributor.authorBOGUEN OJEDA, RODRIGO ESTEBAN
dc.date.accessioned2025-10-06T14:22:11Z
dc.date.available2025-10-06T14:22:11Z
dc.description.abstractPurpose: Ferroptosis is a type of iron-dependent regulated cell death characterized by increased bioavailability of redox-active iron, loss of GPX4 antioxidant capacity, and oxidation of polyunsaturated fatty acid-containing phospholipids mediated by reactive oxygen species (ROS). The aim of this study was to evaluate the effect of oxidative stress induced by arachidonic acid (AA) on ferroptotic cell death in human spermatozoa. Materials and Methods: Spermatozoa from normozoospermic donors were exposed to AA (5, 25, and 50 ?M) for 1 hour at 37 °C, including an untreated control. Oxidative stress was confirmed by evaluation of cytosolic and mitochondrial ROS production, viability, mitochondrial membrane potential (??m) and motility. Subsequently, molecular markers of ferroptosis including iron content, levels of GPX4, SLC7A11, ACSL4, IREB2 and lipid peroxidation were evaluated. The analyses were carried out using either flow cytometry, a microplate reader or confocal laser microscopy. Results: AA-induced oxidative stress showed increased cytosolic and mitochondrial ROS production accompanied by impaired ??m, viability and motility in human spermatozoa. These results were associated with biochemical and molecular markers related to ferroptotic cell death including an increase in iron content in the form of ferrous (Fe2+) ions, SLC7A11, ACSL4, IREB2, a decrease in the level of GPX4, and an increase in the level of lipid peroxidation compared to the untreated control. Conclusions: This study revealed that AA-induced oxidative stress induces cell death with biochemical characteristics of ferroptosis in human spermatozoa, demonstrating another mechanism of alteration of sperm function induced by oxidative stress and could establish new therapeutic objectives to prevent the decrease in sperm quality mediated by oxidative stress. © 2025 Elsevier B.V., All rights reserved.
dc.description.ia_keywordoxidative, stress, spermatozoa, ferroptosis, iron, cell, death
dc.formatPDF
dc.identifier.issn2287-4690
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6969
dc.language.isoen
dc.publisherKorean Society For Sexual Medicine And Andrology
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceWorld Journal of Men'S Health
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2024
oaire.citationTitleWorld Journal of Men'S Health
oaire.citationVolume42
oaire.fundingReferenceANID FONDECYT 1230410, 11200955, 11201011, 3210593, 3230085
oaire.fundingReferenceUFRO PP23-0052
oaire.fundingReferenceANID PFCHA DOCTORADO NACIONAL 21212091
oaire.fundingReferenceCEBIOR (APOYO MATERIAL LABORATORIO)
oaire.licenseConditionObra bajo licencia Creative Commons Atribución-No Comercial 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by-nc/4.0/
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublicationc9b8540f-e025-4d57-95dd-0de22ec54fa0
relation.isAuthorOfPublication.latestForDiscoveryc9b8540f-e025-4d57-95dd-0de22ec54fa0
uct.catalogadorjvu
uct.comunidadCiencias de la Saluden_US
uct.departamentoDepartamento de Procesos Diagnósticos y Evaluación
uct.facultadFacultad de Ciencias de la Salud
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed Central (PMC)
uct.indizacionPubMed
uct.indizacionEmbase
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