The voltage-gated T-type Ca2+ channel is key to the sperm motility of Atlantic salmon (Salmo salar)

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datacite.alternateIdentifier.citationFish Physiology and Biochemistry, 46 (5), 1831-1825, 2020
datacite.alternateIdentifier.doi10.1007/s10695-020-00829-1
datacite.alternateIdentifier.issn0920-1742
datacite.creatorBeltran, Jorge F.
datacite.creatorBelén, Lisandra Herrera
datacite.creatorLee-Estevez, Manuel
datacite.creatorVillalobos, Elías Figueroa
datacite.creatorDumorné, Kelly
datacite.creatorFarias, Jorge G.
datacite.date2020
datacite.rightsRegistro bibliográfico
datacite.subjectIn Silico
datacite.subjectMotility
datacite.subjectNnc 55-0396
datacite.subjectSalmo Salar
datacite.subjectVoltage-gated Ca2+ Channels T-type
datacite.subjectBenzimidazoles
datacite.subjectCalcium Channels, T-type
datacite.subjectCyclopropanes
datacite.subjectNaphthalenes
datacite.subjectNnc 55-0396
datacite.subjectBenzimidazole Derivative
datacite.subjectCalcium Channel T Type
datacite.subjectCyclopropane Derivative
datacite.subjectNaphthalene Derivative
datacite.subjectNnc 55-0396
datacite.subjectAnimal
datacite.subjectDrug Effect
datacite.subjectMale
datacite.subjectMetabolism
datacite.subjectMolecular Model
datacite.subjectPhysiology
datacite.subjectProtein Conformation
datacite.subjectSalmo Salar
datacite.subjectSpermatozoon
datacite.subjectSpermatozoon Motility
datacite.subjectAnimals
datacite.subjectBenzimidazoles
datacite.subjectCalcium Channels, T-type
datacite.subjectCyclopropanes
datacite.subjectMale
datacite.subjectModels, Molecular
datacite.subjectNaphthalenes
datacite.subjectProtein Conformation
datacite.subjectSperm Motility
datacite.subjectSpermatozoa
datacite.titleThe voltage-gated T-type Ca2+ channel is key to the sperm motility of Atlantic salmon (Salmo salar)
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorFARIAS VEGA, CRISTIAN ALEX
dc.description.abstractCa2+ is a key element in the sperm activation process of Salmo salar. However, the molecular mechanisms by which this ion enters the sperm cell have been poorly studied. In this study, we examined, for the first time, the role of the voltage-gated T-type Ca2+ channel in the activation of sperm motility of Salmo salar. Using an in vitro inhibition assay, a significant decrease in total and progressive motility (P < 0.0001) was observed in Salmo salar sperm when they were treated with NNC-55-0396, a highly selective blocker. The in silico analysis showed that this blocker is docked with a strong affinity for the pore of the voltage-gated T-type calcium channel suggesting the blocking of Ca2+ ions. The results show that the T-type voltage-gated Ca2+ channel is key to sperm motility in Salmo salar. © 2020 Elsevier B.V., All rights reserved.
dc.description.ia_keywordsperm, salmo, salar, voltage, gated, type, channel
dc.identifier.issn1573-5168
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3424
dc.language.isoen
dc.publisherSpringer Nature
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceFish Physiology and Biochemistry
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2020
oaire.citationEndPage1831
oaire.citationIssue5
oaire.citationStartPage1825
oaire.citationTitleFish Physiology and Biochemistry
oaire.citationVolume46
oaire.fundingReferenceANID FONDECYT 1180387 (Regular)
oaire.fundingReferenceUFRO DIUFRO DI12-PEO1, DIE14-0001
oaire.fundingReferenceUFRO PhD Scholarship
oaire.licenseConditionCopyright © Springer Nature, 2020
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
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relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Biológicas y Químicas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionScimago
uct.indizacionPubMed
uct.indizacionEMBASE
uct.indizacionZoological Record
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