Role of three plasma membrane Ca2+-binding proteins in the sperm motility of Atlantic salmon (Salmo salar)
- Beltran, Jorge F. - Figueiras-Fierro, D. - Zamorano, Mauricio - Herrera Belen, Lisandra - Risopatron, Jennie - Valdebenito, Ivan - Norambuena, Juan-Alejandro - Farias, Jorge G.
- Datos de publicación:
- Salmo salar - Motility - In vitro - In silico - Channel -
- Migración Web of Science 
- Salmonid sperm motility is regulated by different factors, such as ion concentration, osmotic pressure, pH, and temperature. The Ca2+ cation-dependent regulation and Ca2+-binding proteins are key regulatory mechanisms in the sperm activation process. In this study, the function of transient receptor potential ankyrin 1, transient receptor potential cation channel subfamily V member 4, and Na+/Ca2+ exchanger 1 on the sperm motility of Salmo salar were studied in vitro and in silico. Sperm inhibition assay results showed that the inhibition of the Na+/Ca2+ exchanger produced a significant decrease in total and progressive motility (p < 0.0001). The molecular docking-based analysis showed that inhibitor KB-R7943 binds strongly (-6.0 kcal/mol) in the Ca2+binding region in this exchanger. Additionally, it was demonstrated that, in contrast to Na+/Ca2+ exchange, neither transient receptor potential ankyrin 1 nor transient receptor potential cation channel subfamily V member 4 was directly associated with sperm motility. This research provides new and useful insights into the role of Ca2+-binding proteins present in the plasma membrane that regulate the sperm motility of Salmo salar.