Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility
Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility
Authors
Troncoso-Ortega, Eduardo
Castillo, Rosario del P.
Reyes-Contreras, Pablo
Castano-Rivera, Patricia
Teixeira Mendonca, Regis
Schiappacasse, Nicolas
Parra, Carolina
Castillo, Rosario del P.
Reyes-Contreras, Pablo
Castano-Rivera, Patricia
Teixeira Mendonca, Regis
Schiappacasse, Nicolas
Parra, Carolina
Profesor GuĆa
Authors
Date
Datos de publicaciĆ³n:
10.3390/biom11040507
BIOMOLECULES,Vol.11,,2021
BIOMOLECULES,Vol.11,,2021
Tipo de recurso
Article
Keywords
Materia geogrƔfica
Collections
Abstract
The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S-0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S-0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.