Ovulation-inducing factor (OIF/NGF) from seminal plasma origin enhances Corpus Luteum function in llamas regardless the preovulatory follicle diameter
Ovulation-inducing factor (OIF/NGF) from seminal plasma origin enhances Corpus Luteum function in llamas regardless the preovulatory follicle diameter
Authors
Silva Jiménez, Mauricio
Ulloa Leal, C.
Norambuena, C.
Fernandez, A.
Adams, G. P.
Ratto, Marcelo
Ulloa Leal, C.
Norambuena, C.
Fernandez, A.
Adams, G. P.
Ratto, Marcelo
Authors
Date
Datos de publicación:
10.1016/j.anireprosci.2014.05.012
Keywords
Collections
Abstract
Ovulation-inducing factor (OIF) is a protein present in llama seminal plasma that has recently been identified as beta-Nerve Growth Factor (NGF) and it induces not only a high rate of ovulation but also appears to have luteotrophic properties in this species. A 2-by-2 experimental design was used to determine the effect of treatments (OIF/NGF vs GnRH) and categories of preovulatory follicle diameter (7-10 vs >10 mm) on ovulation rate, CL diameter and function in llamas. Llamas (n = 32 llamas per group) were randomly assigned to receive an intramuscular dose of: (a) 1 mg purified OIF/NGF in the presence of a follicle of 7-10 mm in diameter: (b) 50 mu g of GnRH in the presence of a follicle of 7-10mm in diameter: (c) 1 mg purified OIF/NGF in the presence of a follicle >10 ram in diameter; (d) 50 mu g of GnRH in the presence of a follicle >10 mm in diameter. Llamas were examined by ultrasonography every 12h from treatment to Day 2 (Day 0 = treatment) to detect ovulation, and again on Day 8 to determine CL diameter. Ovulation rates did not differ among groups. There was an effect of preovulatory follicle size on Corpus Luteum diameter at Day 8 (P < 0.001), however plasma progesterone concentration (n = 15/per group) was higher (P < 0.05) in the OIF/NGF - than that of the GnRH - treated group by the same day. We conclude that OIF/NGF treatment enhances CL function regardless preovulatory follicle size at the time of treatment. (C) 2014 Elsevier B.V. All rights reserved.