Arcobacter butzleri survives within trophozoite of Acanthamoeba castellanii
Arcobacter butzleri survives within trophozoite of Acanthamoeba castellanii
Authors
Villanueva, Maria P.
Medina Schwerter, Gustavo
Fernandez, Heriberto
Medina Schwerter, Gustavo
Fernandez, Heriberto
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Date
Datos de publicaciĆ³n:
10.1016/j.ram.2015.12.003
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Abstract
The survival of three Arcobacter butzleri strains inside Acanthamoeba castellanii was assessed using axenic cultures of A. castellanii that were inoculated with the tested strains and incubated at 26 degrees C under aerobic conditions for 240 h. The behavior of bacteria in contact with amoebae was monitored using phase contrast microscopy. The bacterial survival rate within amoebae was assessed through counting colony forming units, using the gentamicin protection assay. All A. butzleri strains were able to survive during 240 h within the amoebae, thus suggesting that (i) A. butzleri resists the amoebic digestion processes at least for the analyzed time; (ii) that A. castellanii could serve as an environmental reservoir for this bacterium, probably acting as a transmission vehicle for A. butzleri. (C) 2016 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L.U.