Efecto de la escarificación y estratificación sobre la germinación in vitro de Aristotelia chilensis (Molina) Stuntz
Efecto de la escarificación y estratificación sobre la germinación in vitro de Aristotelia chilensis (Molina) Stuntz
Authors
Rodríguez Beraud, Mario
Tampe Perez, Jocelyne
Hormazabal Vasquez, Nelson
Araneda Durán, Ximena
Tighe Neira, Ricardo
Cárcamo Fincheira, Paz
Tampe Perez, Jocelyne
Hormazabal Vasquez, Nelson
Araneda Durán, Ximena
Tighe Neira, Ricardo
Cárcamo Fincheira, Paz
Authors
Date
Datos de publicación:
10.4067/S0717-66432017000200282
Keywords
Propagación - Semillas - Dormancia
Collections
Abstract
Con el fin de mejorar la germinación de semillas de Aristotelia chilensis, se establecieron dos ensayos in vitro. En el primero, se realizó escarificación y se comparó con un testigo. En el segundo, se aplicó estratificación fría por diferentes períodos de tiempo (2, 4, 6 y 8 semanas a 4 °C en oscuridad), más un testigo sin estratificar. Las semillas fueron germinadas en placas con agua y agar (AA) e incubadas por 60 días en cámara de cultivo a 25 ± 2 °C, con tubos fluorescentes de luz fría blanca bajo un fotoperíodo de 16 h luz, 8 h de oscuridad y una intensidad lumínica de 50 μmol m-2 s-1. En todos los tratamientos se logró valores promedios de germinación superiores a los testigos. La mayor germinación se obtuvo con escarificación (Gmáx, 92%), no obstante la mayor velocidad inicial de germinación se registró con la estratificación por ochomáx semanas, alcanzando el menor tiempo medio de germinación (TM, 18 días). Los resultados demuestran que la semilla de A. chilensis presenta dormancia primaria de nivel moderado tanto exógena como endógena.
Two in vitro assays were performing in order to improve Aristotelia chilensis germination rate. Scarification was tested in the first assay and compare to a control. In the second assay, cold stratification was applied during 2, 4, 6 and 8 weeks at 4 °C in darkness, plus an unstratified control. Seeds were germinated on Petri dish containing water and agar (WA), and incubated for 60 days in a controlled atmosphere at 25 ± 2 °C using cold white fluorescent light tubes (50 μmol m-2 s-1 light intensity), under a photoperiod of 16 h light and 8 h of darkness. All treatments achieved higher average germination rates than the control. The highest germination rate was obtained with scarification (Gmáx, 92%) though the fastest growth was achieved with 8 weeks of stratification, reaching the lowest average germination time of 18 days. The results demonstrate that A. chilensis seeds present a moderate level of primary dormancy, both exogenous and endogenous.
Two in vitro assays were performing in order to improve Aristotelia chilensis germination rate. Scarification was tested in the first assay and compare to a control. In the second assay, cold stratification was applied during 2, 4, 6 and 8 weeks at 4 °C in darkness, plus an unstratified control. Seeds were germinated on Petri dish containing water and agar (WA), and incubated for 60 days in a controlled atmosphere at 25 ± 2 °C using cold white fluorescent light tubes (50 μmol m-2 s-1 light intensity), under a photoperiod of 16 h light and 8 h of darkness. All treatments achieved higher average germination rates than the control. The highest germination rate was obtained with scarification (Gmáx, 92%) though the fastest growth was achieved with 8 weeks of stratification, reaching the lowest average germination time of 18 days. The results demonstrate that A. chilensis seeds present a moderate level of primary dormancy, both exogenous and endogenous.