ANTIMUTAGENIC ACTIVITY OF BUDDLEJA GLOBOSA EXTRACTS IN THE DROSOPHILA WING-SPOT TEST

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Autor:
Carmona Ortíz, Erico - Escobar, Bibi - Obando, Veroska
URI:
http://repositoriodigital.uct.cl/handle/10925/2825
Datos de publicación:
FRESENIUS ENVIRONMENTAL BULLETIN,Vol.25,5758-5764,2016
Temas:
Chile - genotoxicity - matico - SMART assay - somatic mutation
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Resumen:
Buddleja globosa is an endemic shrub species from South America with recognized wound healing, analgesic, anti-inflammatory and antioxidant properties; however, there are not studies about their mutagenicity/antimutagenicity potential. To this end, we used the wing-spot test in Drosophila melanogaster to assess mutagenic and antimutagenic activities of B. globosa. Treatments with different non-toxic concentrations of aqueous extracts from B. globosa leaves did not induce a significant increase in the frequency of any class of mutant spot formation on the wing blades of adult flies. Combined treatments with the mutagenic agent ethyl methane sulphonate (EMS) and leaf extracts showed a relevant reduction of single, large and total mutant spots in somatic wing cells in comparison to those induced by the EMS alone. The results indicate that aqueous extracts of B. globosa show desmutagenic effects in an in vivo model on D. melanogaster. Verbascoside and luteolin are the main bioactive components isolated from B. globosa leaf extracts and these kind of polyphenols could be related with their antimutagenicity. This study is the first in assessing the mutagenic and antimutagenic potential of B. globosa.

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