High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile

Anabalon, Leonardo
Encina Montoya, Francisco
Sanchez, Pamela
Solano Solís, Jaime
Benavente Muñoz, Felipe
Guinez, Basilio
Olivares, Flavio
Oberti Grassau, Carlos
Vega Aguayo, Rolando
Datos de publicación:
ECOLOGY AND EVOLUTION,Vol.9,7448-7454,2019
Easy, economic, precise species authentication is currently necessary in many areas of research and diagnosis in molecular biology applied to conservation studies of endangered species. Here, we present a new method for the identification of three fox species of the Lycalopex genus in Chile. We developed an assay based on high-resolution melt analysis of the mitochondrial cytochrome B gene, allowing a simple, low cost, fast, and accurate species determination. To validate the assay applicability for noninvasive samples, we collected fecal samples in the Atacama Desert, finding unexpectedly one species outside of its known distribution range. We conclude that the assay has a potential to become a valuable tool for a standardized genetic monitoring of the Lycalopex species in Chile.