Metabolomic analyses of highbush blueberry (Vaccinium corymbosum L.) cultivars revealed mechanisms of resistance to aluminum toxicity

dc.contributor.authorCarcamo-Fincheira, Paz
dc.contributor.authorReyes-Diaz, Marjorie
dc.contributor.authorOmena-Garcia, Rebeca P.
dc.contributor.authorVargas, Jonas Rafael
dc.contributor.authorAlvear, Marysol
dc.contributor.authorFlorez-Sarasa, Igor
dc.contributor.authorRosado-Souza, Laise
dc.contributor.authorRengel, Zed
dc.contributor.authorFernie, Alisdair R.
dc.contributor.authorNunes-Nesi, Adriano
dc.contributor.authorInostroza-Blancheteau, Claudio
dc.date2021
dc.date.accessioned2021-10-04T17:39:46Z
dc.date.available2021-10-04T17:39:46Z
dc.description.abstractAluminum (Al) is an important factor that limits plant growth under acidic soil conditions. However, several plant species developed distinct mechanisms that limit the damage caused by high Al concentrations. In highbush blueberry (Vaccinium corymbosum), the Al resistance mechanisms are not fully understood. This study was designed to evaluate the effect of Al toxicity on roots and leaves of highbush blueberry genotypes with contrasting Al resistance [Star (Al-sensitive) and Camellia and Cargo (Al-resistant)] and identify the main molecular and physiological strategies underpinning adaptive Al stress responses in nutrient solution. After 48 h of Al treatment, the reduced form of ascorbate (ASC) was higher in roots, but unchanged in leaves of Cargo and Camellia genotypes compared to the control. We also observed decreased root exudation of oxalate in the Al-treated sensitive cultivar Star throughout the treatment period. However, in the resistant cultivar (Camellia), the exudation of oxalate increased 2.4- and 2.8-fold at 24 and 48 h, respectively. Al treatment differentially affected the enzyme activity and gene expression of the tricarboxylic acid (TCA) cycle enzymes. NAD-dependent malate dehydrogenase (NAD-MDH) expression in roots of cultivar Cargo was reduced at 24 h and increased at 48 h, whereas in leaves the expression was higher at 24 h and decreased at 48 h compared to the control. Citrate synthase (CS) activity in Al-resistant Cargo roots diminished at 24 h, increasing afterwards, without variation in the CS gene expression, compared with the initial time point (t = 0). In Al-resistant Camellia roots, the gene expression and the activity of CS decreased during Al exposure. NADP-dependent malate dehydrogenase (NADP-MDH) activity showed increased activity and gene expression at 24 h, in the leaves of cultivar Cargo, whereas in roots the gene expression decreased, but the activation state of NADP-MDH increased. The expression of genes encoding TCA cycle enzymes did not differ significantly in the Al-sensitive cultivar Star during Al exposure. In conclusion, the exudation of organic acid anions, particularly oxalate, plays an important role in Al resistance of highbush blueberry genotypes whilst elevated levels of ASC in roots, also contribute to the Al-resistance mechanisms exhibited by genotypes Camellia and Cargo.
dc.identifier.citationENVIRONMENTAL AND EXPERIMENTAL BOTANY,Vol.183,,2021
dc.identifier.doi10.1016/j.envexpbot.2020.104338
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/4214
dc.language.isoen
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.sourceENVIRONMENTAL AND EXPERIMENTAL BOTANY
dc.subject.englishTCA cycle
dc.subject.englishOxalate
dc.subject.englishAscorbate
dc.subject.englishOrganic acid anions
dc.titleMetabolomic analyses of highbush blueberry (Vaccinium corymbosum L.) cultivars revealed mechanisms of resistance to aluminum toxicity
dc.typeArticle
uct.indizacionSCI
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