In vitro maturation of cat oocytes obtained from females treated with Follicle Stimulating Hormone

Sanchez Riquelme, Alfonso
Lopez Zamorano, Leonardo
Silva Jimenez, Mauricio
Berland Olea, Marco
Datos de publicación:
The aim of this research was to evaluate the effect of Follicle Stimulating Hormone (FSH) treatment on quality and in vitro maturation potential of cumulus-oocyte complexes (COCs) recovered from domestic cats. Twenty-one mature cats were randomly assigned to two groups, FSH (n=9) and Control (n=12). Cats in FSH group were treated with 5 mg of Folltropin-V subcutaneously every 24 hours, for 4 consecutive days. Cats in Control group received 1 mL of sterile saline solution every 24 hours for 4 days. Ovaries were obtained surgically 24 hours after the end of the gonadotrophic treatment. Ovaries were then transported to the laboratory at 37 degrees C within 4 hours after surgery. COCs were recovered by slicing, and classified according to morphological features. Maturation medium was based on TCM-1199, and supplemented with 0.4% BSA, 1 mu L/mL FSH, 1 mu L/mL LH y 1 mu g/mL of 17 beta oestradiol, 50 mu g/mL gentamicin, 0.20 mM sodium pyruvate. The maturation period was of 24 hours under 38.5 degrees C, 5% CO2 and saturated humidity. After this period oocytes were denuded, fixed and stained to asses their maturational status. Oocytes were considered mature when it was observed the presence of metaphase 11 plate and the expulsion of the first polar body. An average of 10.6 +/- 3.6 and 7.1 +/- 2.8 excellent and good quality COCs were recovered from cats in FSH and Control group respectively (P <= 0.05). Twenty five point six percent (25.6%) and 17% of recovered COCs from FSH and Control group respectively were classified as excellent or good (P :5 0.05). In vitro maturation rate was of 73.6% and 49.4% for the FSH group and the control group respectively (P <= 0.05). It can be concluded that FSH treatment increases the number of excellent and good quality oocytes obtained from cats and that these oocytes present a higher in vitro maturation potencial.