Isolation and molecular characterization of MYB60 in Solanum lycopersicum

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dc.contributor.authorRodriguez hoces de la Guardia, Amparo
dc.contributor.authorUgalde, Maria Beatriz
dc.contributor.authorLobos Diaz, Valeria
dc.contributor.authorRomero Romero, Jesus Lucina
dc.contributor.authorMeyer Regueiro, Carlos
dc.contributor.authorInostroza Blancheteau, Claudio
dc.contributor.authorReyes Diaz, Marjorie
dc.contributor.authorAquea, Felipe
dc.contributor.authorArce Johnson, Patricio
dc.date2021
dc.date.accessioned2021-04-30T17:06:06Z
dc.date.available2021-04-30T17:06:06Z
dc.description.abstractStomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.
dc.identifier.citationMOLECULAR BIOLOGY REPORTS,Vol.48,1579-1587,2021
dc.identifier.doi10.1007/s11033-021-06168-5
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/4020
dc.language.isoen
dc.publisherSPRINGER
dc.sourceMOLECULAR BIOLOGY REPORTS
dc.subject.englishAbiotic stress
dc.subject.englishDrought
dc.subject.englishMYB60
dc.subject.englishTomato
dc.subject.englishStomatal aperture
dc.titleIsolation and molecular characterization of MYB60 in Solanum lycopersicum
dc.typeArticle
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