Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis

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dc.contributor.authorHernández Montelongo, Jacobo
dc.contributor.authorCorrales Urena, Y. R.
dc.contributor.authorMachado, D.
dc.contributor.authorLancelloti, M.
dc.contributor.authorPinheiro, M. P.
dc.contributor.authorRischka, K.
dc.contributor.authorLisboa Filho, P. N.
dc.contributor.authorCotta, M. A.
dc.date2018
dc.date.accessioned2019-11-13T19:29:46Z
dc.date.available2019-11-13T19:29:46Z
dc.date.issued2019-11-13
dc.description.abstractStaphylococcus epidermidis is a gram-positive bacterium, and one of the most prevalent causes of nosocomial infections due to its strong ability to form biofilms on catheters and surgical implants. Here we explore the antimicrobial properties of Tet-124 peptides, which are part of the innate defense against different multicellular organisms in nature. Two different Tet-124 peptides were immobilized on a polyethylenimine (PEI) film to determine their impact on the antimicrobial properties: KLWWMIRRW (Tet-124), which contains only natural amino acids, and KLWWMIRRWG-(F-Br)-G (F-Br- 4-Bromophenylalanine), a modified Tet-124 sequence with the addition of an unnatural amino acid. The immobilization was obtained as a result of the electrostatic interaction between PEI amino groups and the C-terminal carboxylic groups of tryptophan and glycine amino acids of Tet-124 and Tet-124-Br peptides, respectively. The process was monitored and studied by water contact angle, Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Quartz Crystal Microbalance with Dissipation (QCM-D) measurements. The antibacterial effect of our samples against S. epidermis was evaluated by the spread plate counting method, and cytotoxicity was tested using fibroblast cultures. Our results indicate the feasibility to immobilize electrostatically both Tet-124 peptides for biomedical applicationses_ES
dc.formatPDFes_ES
dc.identifier.citationColloids and Surfaces b-Biointerfaces, Vol.164, 370-378, 2018
dc.identifier.doi10.1016/j.colsurfb.2018.02.002es_ES
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2082
dc.language.isoenes_ES
dc.sourceColloids and Surfaces b-Biointerfaceses_ES
dc.subjectElectrostatic immobilizationes_ES
dc.subjectStaphylococcus epidermidises_ES
dc.subjectAntimicrobial peptideses_ES
dc.titleElectrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidises_ES
dc.typeArtículo de Revistaes_ES
uct.catalogadorpopes_ES
uct.facultadFacultad de Ingenieríaes_ES
uct.indizacionISI - Science Citation Indexes_ES
uct.indizacionSCOPUSes_ES
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