Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium

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dc.contributor.authorUlloa Rodriguez, Patricio
dc.contributor.authorContreras, Pablo
dc.contributor.authorDumorne, Kelly
dc.contributor.authorLee Estevez, Manuel
dc.contributor.authorDiaz, Rommy
dc.contributor.authorFigueroa Villalobos, Elías
dc.contributor.authorValdebenito Isler, Iván
dc.contributor.authorRisopatron, Jennie
dc.contributor.authorFarias, Jorge G.
dc.date2018
dc.date.accessioned2021-04-30T16:47:47Z
dc.date.available2021-04-30T16:47:47Z
dc.description.abstractPatagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The aim of this study is to evaluate the ultrastructure and quality of E. maclovinus sperm during refrigerated storage. To address this objective, scanning electron microscopy (SEM), cytofluorimetric analysis (membrane integrity; reactive oxygen species generation; mitochondrial membrane potential) and cell respiration/mitochondrial-function analysis (ATP content; oxygen consumption) could be useful for optimizing or improving management for artificial reproduction of this species. Severe damage of plasma membranes was observed by SEM at day 7 and 14 of in vitro storage. Analyses of sperm quality were conducted during the 14-day cold storage period when sperm were in diluted (with Cortland solution) and undiluted conditions. When there were diluted conditions, there was greater preservation of motile capacity (from day-7; P < 0.05), membrane integrity (from day-7; P < 0.05), mitochondrial membrane potential (from day-10; P < 0.05) and ATP stores (from day-3; P < 0.05). Oxygen consumption indicators were 18.6% +/- 14.7% greater in the undiluted samples from day-3, and 32.1% +/- 2.1% of the total spermatozoa had ample amounts of superoxide anion in both undiluted and diluted semen on day-0. The use of Cortland solution extended the viability of sperm when there were longer storage times. Factors that have a greater effect on the quality of semen during storage are reactive oxygen species generation and ATP depletion. In conclusion, Patagonian blenny spermatozoa can be stored at 4 degrees C between 7 and 10 days using Cortland solution.
dc.identifier.citationANIMAL REPRODUCTION SCIENCE,Vol.197,117-125,2018
dc.identifier.doi10.1016/j.anireprosci.2018.08.019
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3515
dc.language.isoen
dc.publisherELSEVIER
dc.sourceANIMAL REPRODUCTION SCIENCE
dc.subject.englishShort-term storage
dc.subject.englishFish spermatozoa
dc.subject.englishSperm function
dc.subject.englishAquaculture
dc.titlePatagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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