Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function

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dc.contributor.authorParedes, Marco
dc.contributor.authorGonzález Alfaro, Katerina
dc.contributor.authorFigueroa, Jaime
dc.contributor.authorMontiel-Eulefi, Enrique
dc.date2013
dc.date.accessioned2021-04-30T16:43:34Z
dc.date.available2021-04-30T16:43:34Z
dc.description.abstractThe in vitro and in vivo effect of prolactin (PRL) on kidney macrophages from Atlantic salmon (Salmo salar) was investigated under the assumption that PRL stimulates immune innate response in mammals. Kidney macrophages were treated two ways: first, cultured in RPMI 1640 medium containing 10, 25, 50 and 100 ng/mL of PRL and second, isolated from a fish with a PRL-injected dose of 100 ng/Kg. Reduced nitro blue tetrazolium (formazan) was used to produce intracellular superoxide anion. Phagocytic activity of PRL was determined in treated cells by optical microscopy observation of phagocytized Congo red-stained yeast. Kidney lysozyme activity was measured in PRL-injected fish. In vitro and in vivo macrophages treated with PRL presented an enhanced superoxide anion production, elevated phagocytic index and increased phagocytic activity. Treated fish showed higher levels of lysozyme activity in the head kidney compared to the control. These results indicate that PRL-stimulated innate immune response in Atlantic salmon and future studies will allow us to assess the possibility of using PRL as an immunostimulant in the Chilean salmon industry.
dc.identifier.citationFISH PHYSIOLOGY AND BIOCHEMISTRY,Vol.39,1215-1221,2013
dc.identifier.doi10.1007/s10695-013-9777-7
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3346
dc.language.isoen
dc.publisherSPRINGER
dc.sourceFISH PHYSIOLOGY AND BIOCHEMISTRY
dc.subject.englishSalmo salar
dc.subject.englishProlactin
dc.subject.englishPhagocytic activity
dc.subject.englishSuperoxide anion
dc.titleImmunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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