Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry

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dc.contributor.authorTrigo, P.
dc.contributor.authorMerino, O.
dc.contributor.authorFigueroa Villalobos, Elías
dc.contributor.authorValdebenito Isler, Iván
dc.contributor.authorSanchez, R.
dc.contributor.authorRisopatron, J.
dc.date2015
dc.date.accessioned2021-04-30T16:34:20Z
dc.date.available2021-04-30T16:34:20Z
dc.description.abstractThe short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O.mykiss during storage time and its relation to the spawning period are little known. This study was designed to evaluate the effects of in vitro short-term storage on sperm functional parameters in O.mykiss, determined by flow cytometry. Semen samples of the first spawning - undiluted (SSD) and diluted (SD) (Storfish((R)) 1:2v/v; IMV AI solutions, France) - were stored at 4 degrees C for 14days. Motility, viability (PMI: plasma membrane integrity) and mitochondrial membrane potential (M) were assessed. On the fifth day of storage, spermatozoa showed a motility >70% (SSD: 78.3% versus SD 85.0%), PMI (81.5% SSD/87.2% SD) and M (72.5% SSD/SD 80.0%) (P<0.05). However, a significant decline in the percentage of all functional parameters (P<0.05) was observed after 5days of storage for all samples of both undiluted (SSD) and diluted semen. In conclusion, the results here provide new data on O.mykiss sperm quality with respect to in vitro short-term storage evaluated by flow cytometry.
dc.identifier.citationANDROLOGIA,Vol.47,407-411,2015
dc.identifier.doi10.1111/and.12276
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3048
dc.language.isoen
dc.publisherWILEY
dc.sourceANDROLOGIA
dc.subject.englishFish
dc.subject.englishflow cytometry
dc.subject.englishmembrane
dc.subject.englishshort-term storage
dc.subject.englishsperm function
dc.titleEffect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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