Increased light intensity during in vitro culture improves water loss control and photosynthetic performance of Castanea sativa grown in ventilated vessels

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dc.contributor.authorSaez, Patricia L.
dc.contributor.authorBravo, Leon A.
dc.contributor.authorLatsague Vidal, Mirtha
dc.contributor.authorSanchez, Manuel E.
dc.contributor.authorRios, Darcy G.
dc.date2012
dc.date.accessioned2021-04-30T16:47:01Z
dc.date.available2021-04-30T16:47:01Z
dc.description.abstractThe effects of in vitro environmental conditions, ventilation of culture vessels and light level, on water loss control and photosynthetic capacity of Castanea swim during in vitro culture were assessed. C. sativa microshoots were cultured in ventilated (V) and non ventilated (NV) vessels, using two photon flux density (PFD) levels, 50 and 150 mu mol photons m(-2) s(-1) (L-50 and L-150, respectively). The use of ventilation and the increase in irradiance improved the performance of the microshoots with respect to traditional in vitro culture of C. sativa (NVL50). Microshoots grown under VL150 showed an increase in stomatal density and improved their functional characteristics, showing a more elliptical shape and lower percentage of open stomata. This paralleled a significantly lower transpiration rate and stomatal conductance. Increasing light level and using ventilated vessels increased the microshoots' capacity to harmlessly dissipate excess absorbed energy, water use efficiency and photosynthetic activity, resulting in a greater production of new microshoots. These improvements during in vitro culture generate microshoots with anatomical and functional characteristics similar to those observed in seedlings, which could help reduce the stress observed during ex vitro transfer. (C) 2012 Elsevier B.V. All rights reserved.
dc.identifier.citationSCIENTIA HORTICULTURAE,Vol.138,7-16,2012
dc.identifier.doi10.1016/j.scienta.2012.02.005
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3500
dc.language.isoen
dc.publisherELSEVIER
dc.sourceSCIENTIA HORTICULTURAE
dc.subject.englishMicropropagation
dc.subject.englishPhoton flux density
dc.subject.englishVentilation
dc.subject.englishTranspiration rate
dc.subject.englishPhotosynthesis
dc.titleIncreased light intensity during in vitro culture improves water loss control and photosynthetic performance of Castanea sativa grown in ventilated vessels
dc.typeArticle
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