Tamoxifen induces apoptotic neutrophil efferocytosis in horses

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dc.contributor.authorOlave, C.
dc.contributor.authorMorales, N.
dc.contributor.authorUberti, B.
dc.contributor.authorHenriquez, C.
dc.contributor.authorSarmiento, J.
dc.contributor.authorOrtloff Trautmann, Alexander
dc.contributor.authorFolch, H.
dc.contributor.authorMoran, G.
dc.date2018
dc.date.accessioned2020-07-09T21:42:33Z
dc.date.available2020-07-09T21:42:33Z
dc.description.abstractMacrophages and neutrophils are important cellular components in the process of acute inflammation and its subsequent resolution, and evidence increasingly suggests that they play important functions during the resolution of chronic, adaptive inflammatory processes. Exacerbated neutrophil activity can be harmful to surrounding tissues; this is important in a range of diseases, including allergic asthma and chronic obstructive pulmonary disease in humans, and equine asthma (also known as recurrent airway obstruction (RAO). Tamoxifen (TX) is a non-steroidal estrogen receptor modulator with effects on cell growth and survival. Previous studies showed that TX treatment in horses with induced acute pulmonary inflammation promoted early apoptosis of blood and BALF neutrophils, reduction of BALF neutrophils, and improvement in animals’ clinical status. The aim of this study was to describe if TX induces in vitro efferocytosis of neutrophils by alveolar macrophages. Efferocytosis assay, myeloperoxidase (MPO) detection and translocation phosphatidylserine (PS) were performed on neutrophils isolated from peripheral blood samples from five healthy horses. In in vitro samples from heathy horses, TX treatment increases the phenomenon of efferocytosis of peripheral neutrophils by alveolar macrophages. Similar increases in supernatant MPO concentration and PS translocation were observed in TX-treated neutrophils, compared to control cells. In conclusion, these results confirm that tamoxifen has a direct effect on equine peripheral blood neutrophils, through stimulation of the engulfment of apoptotic neutrophils by alveolar macrophagesen_US
dc.formatPDFen_US
dc.identifier.citationVeterinary Research Communications, Vol.42, N°1, 57-63en_US
dc.identifier.doi10.1007/s11259-017-9709-6en_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2264
dc.language.isoenen_US
dc.rightsObra bajo licencia Creative Commons 3.0en_US
dc.sourceVeterinary Research Communications, Vol.42, N°1, 57-63en_US
dc.subjectEfferocitosis-Caballosen_US
dc.subjectEquine asthmaen_US
dc.subjectNeutrophilsen_US
dc.titleTamoxifen induces apoptotic neutrophil efferocytosis in horsesen_US
dc.typeArtículo de Revistaen_US
uct.catalogadormlmen_US
uct.comunidadRecursos Naturalesen_US
uct.disciplinaMedicina Veterinaria (General)en_US
uct.indizacionSCOPUSen_US
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