Perforated Patch Clamp in Non-Neuronal Cells, the Model of Mammalian Sperm Cells

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URI:
https://hdl.handle.net/10925/906
Carrera:
Medicina Veterinaria
Facultad:
Facultad de Recursos Naturales
Fecha de publicación:
2012-04-26
Datos de publicación:
InTech, 2012
Temas:
Bioquímica - Genética - Biología Molecular
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Resumen:
This chapter deals with the steps required to obtain perforated patch-clamp recordings from mammalian sperm cells. (in spite of the fact that these cells) In spite of the fact that these cells are not electrically excitable, they possess a number of conductances due to their expression of ion channels and even neurotransmitter-gated ion-channels, many of which remain to be explored functionally and structurally. Detailed methods for obtaining cells suitable for electrophysiological recordings and protocols to perform patch-clamp recordings are outlined in the text.

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