Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster
Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster
Authors
Carmona Ortíz, Erico
Inostroza Blancheteau, Claudio
Obando, Veroska
Rubio, Laura
Marcos, Ricard
Inostroza Blancheteau, Claudio
Obando, Veroska
Rubio, Laura
Marcos, Ricard
Authors
Date
Datos de publicación:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,Vol.791,1-11,2015
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Abstract
Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions. (C) 2015 Elsevier B.V. All rights reserved.